Team:Tsinghua-E/Project

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<li><a href="https://2013.igem.org/Team:Tsinghua-E/Project">Overview</a></li>
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This is a template page. READ THESE INSTRUCTIONS.
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        <li><a href="https://2013.igem.org/Team:Tsinghua-E/Result">Results</a></li>
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        <li><a href="https://2013.igem.org/Team:Tsinghua-E/Film">Film</a></li><br/>
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        <li><a href="/Team:Tsinghua-E/Safety">Safety</a></li>
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.
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<h2>Overview</h2>
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<h3 align="center"><strong>Darwinian evolution for microbial cell factory:</strong><br />
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<em>in vivo</em> evolution engineering towards tryptophan-overproduction superbug</h3>
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Darwinian evolution shows great power in creating incredible biological function in amazing speed.Inspired by this, our team aimed at creating novel fast and irrational microbial cell factory by simulating natural Darwinian evolution process. With tryptophan as target product, a novel tryptophan biosensor utilizing translating ribosome mechanism was firstly developed as the foundation for tryptophan productivity and selection pressure switch module. We further constructed this tryptophan overproduction selection gene circuit coupling with in vivo mutation machine (mutator gene of mutD). By fine-tuning the selection conditions, our selection circuit showed good tryptophan dependent growth property, which provides the foundation for further evolution.As a preliminary result of this project, we successfully evolved an ancestor with zero productivity to a high-tryptophan producer only after several rounds of evolution.
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:Tsinghua-E|Home]]
 
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!align="center"|[[Team:Tsinghua-E/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Tsinghua-E Official Team Profile]
 
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!align="center"|[[Team:Tsinghua-E/Project|Project]]
 
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!align="center"|[[Team:Tsinghua-E/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Tsinghua-E/Modeling|Modeling]]
 
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!align="center"|[[Team:Tsinghua-E/Notebook|Notebook]]
 
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!align="center"|[[Team:Tsinghua-E/Safety|Safety]]
 
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!align="center"|[[Team:Tsinghua-E/Attributions|Attributions]]
 
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== '''Overall project''' ==
 
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Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)
 
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== Project Details==
 
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=== Part 2 ===
 
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=== The Experiments ===
 
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=== Part 3 ===
 
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== Results ==
 

Latest revision as of 09:53, 10 October 2013

  • Totop

















Overview

Darwinian evolution for microbial cell factory:
in vivo evolution engineering towards tryptophan-overproduction superbug

Darwinian evolution shows great power in creating incredible biological function in amazing speed.Inspired by this, our team aimed at creating novel fast and irrational microbial cell factory by simulating natural Darwinian evolution process. With tryptophan as target product, a novel tryptophan biosensor utilizing translating ribosome mechanism was firstly developed as the foundation for tryptophan productivity and selection pressure switch module. We further constructed this tryptophan overproduction selection gene circuit coupling with in vivo mutation machine (mutator gene of mutD). By fine-tuning the selection conditions, our selection circuit showed good tryptophan dependent growth property, which provides the foundation for further evolution.As a preliminary result of this project, we successfully evolved an ancestor with zero productivity to a high-tryptophan producer only after several rounds of evolution.


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