Team:Cornell/project/wetlab/fungal toolkit

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<h6>Dry Lab</h6>
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<a href="https://2012.igem.org/Team:Cornell/project/drylab">How It Works</a>
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<a href="https://2012.igem.org/Team:Cornell/project/drylab/functional_requirements">Functional Requirements</a>
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<a href="https://2012.igem.org/Team:Cornell/project/drylab/components">Components</a>
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Modeling
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<h2 class="centered">How It Works</h2>
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<h2 class="centered">Fungal Toolkit</h2>
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<b>Scroll over each number to find out more about the components of our device!</b>
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<b>Scroll over each icon to find out more about the components of our toolkit!</b>
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<h5>Inlet</h5>
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Water flows into our system, providing a sample to test water quality.
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<h5>Regulatory Elements</h5>
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Provide modular protein expression controls for genetic constructs.
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<h5>Filter</h5>
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Prevents foreign microbes from entering and contaminating our reactor.
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Move water and food to and from the reactor.
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<h5>Selectable Markers</h5>
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Allow for the selection of desired fungal transformants.
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Resist corrosion and set flow rates to the optimal level.
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<h5>Reactors</h5>
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House bacteria that produce electrical current in response to toxins.
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<h5>Characterization</h5>
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Quantifies gene expression levels using fluorescent proteins.
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Supply our bacteria with lactate to keep them alive.
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Record bacterial current and transmit data wirelessly to the user.
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<h5>Homologous Constructs</h5>
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Enable integration of genetic constructs into specific regions of fungal genomes.
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Keeps our device running.
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Extends the longevity of our device by providing extra power.
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Implements measures to prevent horizontal gene transfer and induce a kill switch.
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Prevents our microbes from leaving the device.
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Water flows back to the source.
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Keeps the device safe from weather, rocks, and, of course, bears.
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<h5>The Full Device</h5>
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In all of its glory.
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Latest revision as of 13:01, 12 October 2013

Cornell University Genetically Engineered Machines

Fungal Toolkit

Scroll over each icon to find out more about the components of our toolkit!
Regulatory Elements
Provide modular protein expression controls for genetic constructs.
Selectable Markers
Allow for the selection of desired fungal transformants.
Characterization
Quantifies gene expression levels using fluorescent proteins.
Homologous Constructs
Enable integration of genetic constructs into specific regions of fungal genomes.
Biosafety
Implements measures to prevent horizontal gene transfer and induce a kill switch.

Retrieved from "http://2013.igem.org/Team:Cornell/project/wetlab/fungal_toolkit"