Team:Cornell/project/wetlab/fungal toolkit

From 2013.igem.org

(Difference between revisions)
 
(19 intermediate revisions not shown)
Line 9: Line 9:
<div class="two columns"></html>{{:Team:Cornell/templates/wetnav}}<html></div>
<div class="two columns"></html>{{:Team:Cornell/templates/wetnav}}<html></div>
<div class="ten columns team-bios-container">
<div class="ten columns team-bios-container">
-
<div class="row"">
+
<div class="row">
<div class="twelve columns">
<div class="twelve columns">
<h2 class="centered">Fungal Toolkit</h2>
<h2 class="centered">Fungal Toolkit</h2>
Line 16: Line 16:
<div class="row last-ele">
<div class="row last-ele">
<div class="twelve columns">
<div class="twelve columns">
-
<b>Scroll over each icon to find out more about the components of our toolkit!</b>
+
<b>Scroll over each icon to find out more about the components of our toolkit!</b>
-
</div>
+
</div>
<div class="row last-ele overflow" style="margin:0px">
<div class="row last-ele overflow" style="margin:0px">
<div class="twelve columns centered overflow no-pad" style="position:relative;">
<div class="twelve columns centered overflow no-pad" style="position:relative;">
<div class="circle-position">
<div class="circle-position">
-
<div class="circle-hover" style="position:absolute;left:266px;top:31px"></div>
+
<a class="circle-hover" style="position:absolute;left:266px;top:31px" href="https://2013.igem.org/Team:Cornell/project/wetlab/fungal_toolkit/regulatory_elements">
-
<div class="circle-hover-pic" style="position:absolute;left:0px;top:0px">
+
<div class="circle-hover">
-
<div class="circle-desc">
+
</div>
-
<h5>Regulatory Elements</h5>
+
</a>
-
Provide modular protein expression controls for genetic constructs.
+
<div class="circle-hover-pic" style="position:absolute;left:0px;top:0px">
 +
<div class="circle-desc">
 +
<h5>Regulatory Elements</h5>
 +
Provide modular protein expression controls for genetic constructs.
 +
</div>
 +
<img class="inline" src="https://static.igem.org/mediawiki/2013/7/7d/CornellCirclesPromoter.png" style="height:760px">
</div>
</div>
-
<img class="inline" src="https://static.igem.org/mediawiki/2013/7/7d/CornellCirclesPromoter.png" style="height:760px">
 
</div>
</div>
-
</div>
+
 
<div class="circle-position">
<div class="circle-position">
-
<div class="circle-hover" style="position:absolute;left:501px;top:202px">
+
<a class="circle-hover" style="position:absolute;left:501px;top:202px" href="https://2013.igem.org/Team:Cornell/project/wetlab/fungal_toolkit/selectable_markers">
-
<img src="https://static.igem.org/mediawiki/2012/9/91/TransBlock.png">
+
<div class="circle-hover">
-
</div>
+
<img src="https://static.igem.org/mediawiki/2013/9/91/TransBlock.png">
-
<div class="circle-hover-pic" style="position:absolute;left:0px;top:0px">
+
-
<div class="circle-desc">
+
-
<h5>Selectable Markers</h5>
+
-
Allow for the selection of desired fungal transformants.
+
</div>
</div>
-
<img class="inline" src="https://static.igem.org/mediawiki/2013/2/21/CornellCirclesSelection.png" style="height:760px">
+
</a>
-
</div>
+
<div class="circle-hover-pic" style="position:absolute;left:0px;top:0px">
 +
<div class="circle-desc">
 +
<h5>Selectable Markers</h5>
 +
Allow for the selection of desired fungal transformants.
 +
</div>
 +
<img class="inline" src="https://static.igem.org/mediawiki/2013/2/21/CornellCirclesSelection.png" style="height:760px">
 +
</div>
 +
 
</div>
</div>
<div class="circle-position">
<div class="circle-position">
-
<div class="circle-hover" style="position:absolute;left:412px;top:477px"></div>
+
<a class="circle-hover" style="position:absolute;left:412px;top:477px" href="https://2013.igem.org/Team:Cornell/project/wetlab/fungal_toolkit/characterization">
-
<div class="circle-hover-pic" style="position:absolute;left:0px;top:0px">
+
<div class="circle-hover">
-
<div class="circle-desc">
+
-
<h5>Characterization</h5>
+
-
Quantifies gene expression levels using fluorescent proteins.
+
</div>
</div>
-
<img class="inline" src="https://static.igem.org/mediawiki/2013/a/a9/CornellCirclesCharacterization.png" style="height:760px">
+
</a>
-
</div>
+
<div class="circle-hover-pic" style="position:absolute;left:0px;top:0px">
 +
<div class="circle-desc">
 +
<h5>Characterization</h5>
 +
Quantifies gene expression levels using fluorescent proteins.
 +
</div>
 +
<img class="inline" src="https://static.igem.org/mediawiki/2013/a/a9/CornellCirclesCharacterization.png" style="height:760px">
 +
</div>
 +
</div>
</div>
<div class="circle-position">
<div class="circle-position">
-
<div class="circle-hover" style="position:absolute;left:121px;top:427px"></div>
+
<a class="circle-hover" style="position:absolute;left:121px;top:427px" href="https://2013.igem.org/Team:Cornell/project/wetlab/fungal_toolkit/homologous_constructs">
-
<div class="circle-hover-pic" style="position:absolute;left:0px;top:0px">
+
<div class="circle-hover">
-
<div class="circle-desc">
+
-
<h5>Homologous Constructs</h5>
+
-
Enable integration of genetic constructs into specific regions of fungal genomes.
+
</div>
</div>
-
<img class="inline" src="https://static.igem.org/mediawiki/2013/8/89/CornellCirclesRecombination.png" style="height:760px">
+
</a>
-
</div>
+
<div class="circle-hover-pic" style="position:absolute;left:0px;top:0px">
 +
<div class="circle-desc">
 +
<h5>Homologous Constructs</h5>
 +
Enable integration of genetic constructs into specific regions of fungal genomes.
 +
</div>
 +
<img class="inline" src="https://static.igem.org/mediawiki/2013/8/89/CornellCirclesRecombination.png" style="height:760px">
 +
</div>
 +
</div>
</div>
<div class="circle-position">
<div class="circle-position">
-
<div class="circle-hover" style="position:absolute;left:32px;top:202px"></div>
+
<a class="circle-hover" style="position:absolute;left:32px;top:202px" href="https://2013.igem.org/Team:Cornell/project/wetlab/fungal_toolkit/biosafety">
-
<div class="circle-hover-pic" style="position:absolute;left:0px;top:0px">
+
<div class="circle-hover">
-
<div class="circle-desc">
+
-
<h5>Biosafety</h5>
+
-
Implements measures to prevent horizontal gene transfer and induce a kill switch.
+
</div>
</div>
-
<img class="inline" src="https://static.igem.org/mediawiki/2013/a/a4/CornellCirclesBiosafety.png" style="height:760px">
+
</a>
-
</div>
+
<div class="circle-hover-pic" style="position:absolute;left:0px;top:0px">
 +
<div class="circle-desc">
 +
<h5>Biosafety</h5>
 +
Implements measures to prevent horizontal gene transfer and induce a kill switch.
 +
</div>
 +
<img class="inline" src="https://static.igem.org/mediawiki/2013/a/a4/CornellCirclesBiosafety.png" style="height:760px">
 +
</div>
 +
</div>
</div>
<img class="inline" src="https://static.igem.org/mediawiki/2013/9/9c/CornellCirclesDots.png" style="margin:0px">
<img class="inline" src="https://static.igem.org/mediawiki/2013/9/9c/CornellCirclesDots.png" style="margin:0px">
</div>
</div>
</div>
</div>
-
<h3>Protoplasting</h3>
+
-
<h5>Background</h5>
+
-
Transformation of plant and fungal cells is difficult due to their cell walls that block passage of foreign DNA. Protoplasting is the method by which the cells walls of plant and fungal cells are digested to produce cells without cell walls, called protoplasts. Through additional methods, such as electroporation and PEG transformation, DNA can be uptaken by the protoplasts and then regrown into cells containing specific genes.
+
-
<h5>Method</h5>Usually this species is protoplasted using lywallzyme and novozyme. However, these enzymes are exclusively available in China. So, protoplasting was attempted with driselease and glucanex instead. After several attempts, we found that the enzymes are unable to digest the cell wall without also killing the cell. In addition, the Ganoderma mycelium was hard to pellet when centrifuging and made the protoplasting procedure difficult. Thus, <i>Cochliobolus heterostrophus</i> was used instead. Protoplasting was successful using driselease and glucanex and then transformed via PEG solution.   
+
-
<h3>References</h3>
+
-
Sun L, Cai H, Xu W, Hu Y, Gao Y, Lin Z (2001). Efficient Transformation of the Medicinal Mushroom Ganoderma lucidum. Plant Molecular Biology Reporter, 19, 383a-383j.
+
-
<br />
+
-
<br />
+
-
Amir Sharon (ed.), <i>Molecular and Cell Biology Methods for Fungi</i>, Methods in Molecular Biology, vol. 638. Turgeon BG, Condon B, Liu J, Zhang N (2010). Protoplast Transformation of Filamentous Fungi.
+
</div>
</div>
</div>
</div>

Latest revision as of 13:01, 12 October 2013

Cornell University Genetically Engineered Machines

Fungal Toolkit

Scroll over each icon to find out more about the components of our toolkit!
Regulatory Elements
Provide modular protein expression controls for genetic constructs.
Selectable Markers
Allow for the selection of desired fungal transformants.
Characterization
Quantifies gene expression levels using fluorescent proteins.
Homologous Constructs
Enable integration of genetic constructs into specific regions of fungal genomes.
Biosafety
Implements measures to prevent horizontal gene transfer and induce a kill switch.

Retrieved from "http://2013.igem.org/Team:Cornell/project/wetlab/fungal_toolkit"