Team:UC Chile/Overview

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                         <div class="text">Modelling</div>
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                         <div class="text">Results</div>
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                             <li class="pos1 sub_item oculto">Mathematical Model<a href="https://2013.igem.org/Team:UC_Chile/Mathematical Model"></a></li>
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                             <li class="pos1 sub_item oculto">BioBricks<a href="https://2013.igem.org/Team:UC_Chile/BioBricks"></a></li>
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                             <li class="pos2 sub_item oculto">Software<a href="https://2013.igem.org/Team:UC_Chile/Software"></a></li>
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                            <li class="pos2 sub_item oculto">Mathematical Model<a href="https://2013.igem.org/Team:UC_Chile/Mathematical Model"></a></li>
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                             <li class="pos3 sub_item oculto">Wet Lab<a href="https://2013.igem.org/Team:UC_Chile/Wet Lab"></a></li>                          
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                         <div class="text">Human<br>Practices</div>
                         <div class="text">Human<br>Practices</div>
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                             <li class="pos1 sub_item oculto">Home<a href="https://2013.igem.org/Team:UC_Chile/Human Practices"></a></li>
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                            <li class="pos2 sub_item oculto">Synbio web<a href="https://2013.igem.org/Team:UC_Chile/Synbio web"></a></li>
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                         <div class="text">Home</div>
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                         <div class="text">More</div>
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                             <li class="pos1 sub_item ">Overview<a href="https://2013.igem.org/Team:UC_Chile/Overview"></a></li>
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                             <li class="pos1 sub_item oculto">Acknowledgments<a href="https://2013.igem.org/Team:UC_Chile/Acknowledgments"></a></li>
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                             <li class="pos2 sub_item ">Achievements<a href="https://2013.igem.org/Team:UC_Chile/Achievements"></a></li>
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                             <li class="pos2 sub_item oculto">Biosafety<a href="https://2013.igem.org/Team:UC_Chile/Biosafety"></a></li>
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                         <div class="text">Project</div>
                         <div class="text">Project</div>
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                             <li class="pos1 sub_item oculto">Whateversisoma<a href="https://2013.igem.org/Team:UC_Chile/Whateversisome"></a></li>
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                             <li class="pos1 sub_item oculto">Description<a href="https://2013.igem.org/Team:UC_Chile/Description"></a></li>
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                             <li class="pos2 sub_item oculto">Biobricks<a href="https://2013.igem.org/Team:UC_Chile/Biobricks"></a></li>
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                             <li class="pos2 sub_item oculto">Formation<a href="https://2013.igem.org/Team:UC_Chile/Formation"></a></li>
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                             <li class="pos3 sub_item oculto">Biosafety<a href="https://2013.igem.org/Team:UC_Chile/Biosafety"></a></li>
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                             <li class="pos3 sub_item oculto">Targeting<a href="https://2013.igem.org/Team:UC_Chile/Targeting"></a></li>
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                             <li class="pos4 sub_item oculto">Side project<a href="https://2013.igem.org/Team:UC_Chile/Side Project"></a></li>
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                             <li class="pos4 sub_item oculto">Purification<a href="https://2013.igem.org/Team:UC_Chile/Purification"></a></li>
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                            <li class="pos5 sub_item oculto">Disruption<a href="https://2013.igem.org/Team:UC_Chile/Disruption"></a></li>
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                            <li class="pos5 sub_item oculto">In vitro Channeling<a href="https://2013.igem.org/Team:UC_Chile/In vitro Channeling"></a></li>                           
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                         <div class="text">Methodology</div>
                         <div class="text">Methodology</div>
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                             <li class="pos1 sub_item oculto">Protocols<a href="https://2013.igem.org/Team:UC_Chile/Protocols"></a></li>
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                             <li class="pos1 sub_item oculto">Lab Notebook<a href="https://2013.igem.org/Team:UC_Chile/Lab NoteBook"></a></li>
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                         <div class="text">More</div>
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                         <div class="text">Home</div>
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                             <li class="pos1 sub_item oculto">Collaborations<a href="https://2013.igem.org/Team:UC_Chile/Collaborations"></a></li>
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                             <li class="pos1 sub_item oculto">Overview<a href="https://2013.igem.org/Team:UC_Chile/Overview"></a></li>
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                <h1>Overview</h1>
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    <h1>Overview</h1>
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                    We are working towards reengineering the Carboxysome, a bacterial microcompartment (BMC), to produce a genetically encoded platform for optimized packaging of metabolic processes and/or enclosed protein production systems.<br>
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In order to do so, we are developing new biobricks for targeting any protein of interest to the inside of Carboxysomes. We are estimating the structure and volume of the Carboxysome to assess and maximize production yields. Moreover, we are developing a novel bacterial microcompartment isolation method by adapting existing technologies to easily purify reengineered Carboxysomes while maintaining their structural and functional properties.<br>
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Imagine a world where production of different compound could be easily achievable, where industrial problems associated with genetic manipulation are over: there is no more instability in the energy flux of bacteria. Imagine a world where there are no more industrial biosafety issues because recombinant bacteria is no longer needed in situ. This is the world we propose: the world of in vitro production.
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Our project should facilitate novel applications using cellular or acellular systems for the compartmentalization of metabolic engineering reactions as well as providing a toolbox for safe and efficient production of biomolecules in bacterial systems.
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To achieve this, we reengineered the Carboxysome, a bacterial microcompartment (BMC) to use it as a continuous reactor capable of generating any output by having the needed enzyme in its inner space. Make Carboxysome your own synthetic space for the production of anything you want:
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A Whateversisome!
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We have successfully:
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    <li>Characterized the Carboxysome as a tool for Synthetic Biology: we presented the plasmid as a standardized biobrick.</li>
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    <li>Found a targeting sequence to the inside of Carboxysome.</li>
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    <li>Designed an innovative methodology for purification and isolation of BMC.</li>
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    <li>We analyzed the potential combinatorial of Carboxysomes on in vitro channeling.</li>
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    </ul><br>       
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Even more…
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    <li> We are working towards generating step-by-step in vitro reactions.</li>
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Latest revision as of 01:53, 13 October 2013

Wiki-IGEM

Overview

Imagine a world where production of different compound could be easily achievable, where industrial problems associated with genetic manipulation are over: there is no more instability in the energy flux of bacteria. Imagine a world where there are no more industrial biosafety issues because recombinant bacteria is no longer needed in situ. This is the world we propose: the world of in vitro production. To achieve this, we reengineered the Carboxysome, a bacterial microcompartment (BMC) to use it as a continuous reactor capable of generating any output by having the needed enzyme in its inner space. Make Carboxysome your own synthetic space for the production of anything you want: A Whateversisome!
We have successfully:
  • Characterized the Carboxysome as a tool for Synthetic Biology: we presented the plasmid as a standardized biobrick.
  • Found a targeting sequence to the inside of Carboxysome.
  • Designed an innovative methodology for purification and isolation of BMC.
  • We analyzed the potential combinatorial of Carboxysomes on in vitro channeling.

Even more…
  • We are working towards generating step-by-step in vitro reactions.

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