Team:BYU Provo/Notebook/SmallPhage/Summerexp/9.13 Mutagen Concentration Test
From 2013.igem.org
(4 intermediate revisions not shown) | |||
Line 12: | Line 12: | ||
|- valign="top" | |- valign="top" | ||
- | | style="width: | + | | style="width: 22%; background-color: transparent;"| |
<font color="#333399" size="3" font face="Calibri"> | <font color="#333399" size="3" font face="Calibri"> | ||
Line 133: | Line 133: | ||
8) Checking for Plaque Viability 2 (9.25) | 8) Checking for Plaque Viability 2 (9.25) | ||
- | * From the plates made in step 6, we selected more large and small plaques. We followed the same procedure as step 7 in the selection process, except we only made a -2 dilution. In total, we had 21 plaques | + | * From the plates made in step 6, we selected more large and small plaques. We followed the same procedure as step 7 in the selection process, except we only made a -2 dilution. In total, we had 21 plaques that looked really large and 10 plaques that looked really small. |
Line 161: | Line 161: | ||
[[File:5Small.JPG|300px|center]] | [[File:5Small.JPG|300px|center]] | ||
+ | |||
+ | |||
[[File:5Large.JPG|300px|center]] | [[File:5Large.JPG|300px|center]] | ||
Line 169: | Line 171: | ||
[[File:6Small.JPG|300px|center]] | [[File:6Small.JPG|300px|center]] | ||
+ | |||
+ | |||
[[File:6Large.JPG|300px|center]] | [[File:6Large.JPG|300px|center]] | ||
Line 181: | Line 185: | ||
* T7 Wild type: Average plaque diameter of 0.388cm +/- 0.0997cm | * T7 Wild type: Average plaque diameter of 0.388cm +/- 0.0997cm | ||
- | [[ | + | [[File:T7WT927.JPG|300px|center]] |
* T7 Small phage: Average plaque diameter of 0.592cm +/- 0.0735cm | * T7 Small phage: Average plaque diameter of 0.592cm +/- 0.0735cm | ||
- | [[File:S4.JPG| | + | [[File:S4.JPG|300px|center]] |
* T7 Large phage: Average plaque diameter of 0.264cm +/- 0.0867cm. | * T7 Large phage: Average plaque diameter of 0.264cm +/- 0.0867cm. | ||
- | [[File:L8.JPG| | + | [[File:L8.JPG|300px|center]] |
* There were also many plates that seemed like they could have mutant phage, but we have to run further experiments to confirm them. | * There were also many plates that seemed like they could have mutant phage, but we have to run further experiments to confirm them. | ||
Line 195: | Line 199: | ||
'''VI) Conclusion''' | '''VI) Conclusion''' | ||
- | From the average plaque diameters in step 8, we conclude that we have found a smaller phage and a larger phage | + | From the average plaque diameters in step 8, we conclude that we have found a smaller phage and a larger phage! The small phage made larger plaques, while the large phage made smaller plaques. The standard deviation is relatively high, but that is most likely due to the already high variability of plaque sizes. Our next steps will be to take a picture of the small and large T7 phage using an electron microscope and sequence their genomes. |
|} | |} |
Latest revision as of 00:34, 14 October 2013
| ||
|
9.13 Mutagen Concentration Test - Ninth Protocol
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Overnight (The day before) (9.13)
2) Applying the mutagen (9.14)
3) Cesium Chloride Gradients (9.16)
4) Spot Test of Gradient Samples (9.18)
5) Plating to Check for Plaque Size (9.20)
6) Plating to Check for Plaque Size 2 (9.23)
7) Checking for Plaque Viability from Step 5 (9.23)
8) Checking for Plaque Viability 2 (9.25)
2) Applying the mutagen
3) Cesium Chloride Gradients
4) Spot Test of Gradient Samples
5) Plating to Check for Plaque Size
6) Plating to Check for Plaque Size 2
7) Checking for Plaque Viability from Step 5
8) Checking for Plaque Viability 2
VI) Conclusion From the average plaque diameters in step 8, we conclude that we have found a smaller phage and a larger phage! The small phage made larger plaques, while the large phage made smaller plaques. The standard deviation is relatively high, but that is most likely due to the already high variability of plaque sizes. Our next steps will be to take a picture of the small and large T7 phage using an electron microscope and sequence their genomes. |