Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period3/Dailylog

(Difference between revisions)

Revision as of 00:50, 14 October 2013

Small Phage September - October Notebook: October 1 - October 15 Daily Log

Small Phage

March-April

10/1/13 - 10/3/13

JL, LP

Tried to take EM to verify mutant size - did not work very well: phage titer is too low

10/4/13 - 10/6/13

JL, LP

10/7/13

JL

10/8/13

JL

10/9/13

JL, LP

10/10/13

LP

Performed spot tests to estimate the mutant phage titer in 10.8 Characterization of Mutant Phage.

JL

Designed primers for cloning the major and minor capsid proteins into the iGEM registry: BI309 (Xbal) and BI310 (SpeI)

10/11/13

JL, LP

Plated the mutant phage at -7 to verify their phenotypic stability. For specifics, please see 10.8 Characterization of Mutant Phage.

Designed primers for more accurate sequencing: BI319 (160bp upstream from start codon) and BI320 (-160 downstream from minor capsid protein stop codon)

10/12/13

JL

10/13/13

JL

Plated more phage from CsCl gradient to select for mutant. Specifics are recorded in 10.8 Characterization of Mutant Phage.

9/27/13

JL, LP

@@ Line 82: / Line 82: @@
 LP
-* Performed a spot to estimate the mutant phage titer in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
+* Performed spot tests to estimate the mutant phage titer in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
 * Started approximately 20 mL of E coli B liquid culture overnight.
+JL
+* Designed primers for cloning the major and minor capsid proteins into the iGEM registry: BI309 (Xbal) and BI310 (SpeI)
 <br>
@@ Line 93: / Line 97: @@
 * Plated the mutant phage at -7 to verify their phenotypic stability. For specifics, please see [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
+* Designed primers for more accurate sequencing: BI319 (160bp upstream from start codon) and BI320 (-160 downstream from minor capsid protein stop codon)
 <br>