Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period3/Dailylog

From 2013.igem.org

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<font size="4"> '''9/16/13''' </font>
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<font size="4"> '''10/1/13 - 10/3/13''' </font>
JL, LP
JL, LP
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* Discussed plans for these future two weeks: established priority.
+
* Worked on putting together Jamboree presentation and poster.  
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* Help Large Phage Group with dilution series and performing a spot test for their small phage band.
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* Tried to take EM to verify mutant size - did not work very well: phage titer is too low
<br>
<br>
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<font size="4"> '''9/17/13''' </font>
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<font size="4"> '''10/4/13 - 10/6/13''' </font>
-
LP
+
JL, LP
-
* Started approximately 20mL of E coli B liquid culture overnight.
+
* North America Regional Jamboree! For more information click [[Team:BYU_Provo/Toronto|here]].
<br>
<br>
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<font size="4"> '''9/18/13''' </font>
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<font size="4"> '''10/7/13''' </font>
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JL, LP
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JL
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* Discussed CsCl gradient set up with Phage Purification Team.
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* Started approximately 10 mL E coli B liquid culture overnight.
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* Performed dilution series and spot test for samples post CsCl from [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
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<br>
 +
 
 +
<font size="4"> '''10/8/13''' </font>
 +
 
 +
JL
 +
 
 +
* Started [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]] by propagating the mutants.
<br>
<br>
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<font size="4"> '''9/19/13''' </font>
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<font size="4"> '''10/9/13''' </font>
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LP
+
JL, LP
 +
 
 +
* Purified the propagated mutant from [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
* Started approximately 20 mL of E coli B liquid culture overnight.
* Started approximately 20 mL of E coli B liquid culture overnight.
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<br>
<br>
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<font size="4"> '''9/20/13''' </font>
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<font size="4"> '''10/10/13''' </font>
LP
LP
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* Performed "Plating to Check for Plaque Size" for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
+
* Performed spot tests to estimate the mutant phage titer in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
-
<br>
+
* Started approximately 20 mL of E coli B liquid culture overnight.
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<font size="4"> '''9/23/13''' </font>
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JL
-
 
+
-
JL, LP
+
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* Performed "Plating to Check for Plaque Size 2" and "Checking for Plaque Viability from Step 5" for  [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
+
* Designed primers for cloning the major and minor capsid proteins into the iGEM registry: BI309 (Xbal) and BI310 (SpeI)
<br>
<br>
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<font size="4"> '''9/24/13''' </font>
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<font size="4"> '''10/11/13''' </font>
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LP
+
JL, LP
 +
 
 +
* Plated the mutant phage at -7 to verify their phenotypic stability. For specifics, please see [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
-
* Started approximately 30 mL E coli B liquid culture overnight.
+
* Designed primers for more accurate sequencing: BI319 (160bp upstream from start codon) and BI320 (-160 downstream from minor capsid protein stop codon)
<br>
<br>
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<font size="4"> '''9/25/13''' </font>
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<font size="4"> '''10/12/13''' </font>
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JL, LP
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JL
-
 
+
-
* Performed "Checking for Plaque Viability 2" for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
+
-
* Started approximately 25mL of E coli B liquid culture overnight
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* Started approximately 15mL of E coli B liquid culture overnight
<br>
<br>
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<font size="4"> '''9/26/13''' </font>
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<font size="4"> '''10/13/13''' </font>
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LP
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JL
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* Started approximately 25mL of E. coli B overnight.
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* Plated more phage from CsCl gradient to select for mutant. Specifics are recorded in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
<br>
<br>
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<font size="4"> '''9/27/13''' </font>
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<font size="4"> '''10/14/13''' </font>
JL, LP
JL, LP
-
* Determined results for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
+
* Plated more phage to look for mutants as part of [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
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+
-
* Finished updating the wiki.
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-
 
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-
* Started approximately 30mL of E. coli B overnight.
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<br>
<br>

Latest revision as of 23:54, 20 October 2013


Small Phage September - October Notebook: October 1 - October 15 Daily Log



Small Phage
March-April
May-June
July-August
September-October

10/1/13 - 10/3/13

JL, LP

  • Worked on putting together Jamboree presentation and poster.
  • Tried to take EM to verify mutant size - did not work very well: phage titer is too low


10/4/13 - 10/6/13

JL, LP

  • North America Regional Jamboree! For more information click here.


10/7/13

JL

  • Started approximately 10 mL E coli B liquid culture overnight.


10/8/13

JL


10/9/13

JL, LP

  • Started approximately 20 mL of E coli B liquid culture overnight.


10/10/13

LP

  • Started approximately 20 mL of E coli B liquid culture overnight.

JL

  • Designed primers for cloning the major and minor capsid proteins into the iGEM registry: BI309 (Xbal) and BI310 (SpeI)


10/11/13

JL, LP

  • Designed primers for more accurate sequencing: BI319 (160bp upstream from start codon) and BI320 (-160 downstream from minor capsid protein stop codon)


10/12/13

JL

  • Started approximately 15mL of E coli B liquid culture overnight


10/13/13

JL


10/14/13

JL, LP


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