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Team:Heidelberg/Templates/Indigoidine week8 overview
From 2013.igem.org
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| - | The PCR screenings showed that the pMM-plasmids are correct. We designed primers to assemble our first [http://parts.igem.org/Part:pSB1C3 pSB1C3] derived plasmid | + | The PCR screenings showed that the pMM-plasmids are correct. We designed primers to assemble our first [http://parts.igem.org/Part:pSB1C3 pSB1C3] derived plasmid pKH1, which carries both the bpsA and the svp gene with the lacI-Promoter [http://parts.igem.org/Part:BBa_R0010?title=Part:BBa_R0010 BBa_R0010] and the ribosome binding site [http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 BBa_B0034] before both the bpsA and the svp gene, respectively.. |
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Latest revision as of 15:07, 21 October 2013
Indigoidine Production - bpsA
We performed PCR screenings of the pMM64 (bpsA) and pMM65 (svp) plasmid for validation.
The PCR screenings showed that the pMM-plasmids are correct. We designed primers to assemble our first [http://parts.igem.org/Part:pSB1C3 pSB1C3] derived plasmid pKH1, which carries both the bpsA and the svp gene with the lacI-Promoter [http://parts.igem.org/Part:BBa_R0010?title=Part:BBa_R0010 BBa_R0010] and the ribosome binding site [http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 BBa_B0034] before both the bpsA and the svp gene, respectively..
