Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period4/Dailylog

Small Phage

March-April

May-June

July-August

September-October

• Plated for mutants as part of 10.8 Characterization of Mutant Phage.

10/18/13

JL, LP

• Performed amplification and purification of insert as part of Cloning T7 Capsid Protein into iGEM Registry.

10/19/13

JL, LP

• Ran a low melt gel for Cloning T7 Capsid Protein into iGEM Registry.

10/20/13

JL

• Performed the repeat PCR and gel step in Cloning T7 Capsid Protein into iGEM Registry

10/9/13

JL, LP

• Purified the propagated mutant from 10.8 Characterization of Mutant Phage.

• Started approximately 20 mL of E coli B liquid culture overnight.

10/10/13

LP

• Performed spot tests to estimate the mutant phage titer in 10.8 Characterization of Mutant Phage.

• Started approximately 20 mL of E coli B liquid culture overnight.

JL

• Designed primers for cloning the major and minor capsid proteins into the iGEM registry: BI309 (Xbal) and BI310 (SpeI)

10/11/13

JL, LP

• Plated the mutant phage at -7 to verify their phenotypic stability. For specifics, please see 10.8 Characterization of Mutant Phage.

• Designed primers for more accurate sequencing: BI319 (160bp upstream from start codon) and BI320 (-160 downstream from minor capsid protein stop codon)

10/12/13

JL

• Started approximately 15mL of E coli B liquid culture overnight

10/13/13

JL

• Plated more phage from CsCl gradient to select for mutant. Specifics are recorded in 10.8 Characterization of Mutant Phage.

9/27/13

JL, LP

• Determined results for 9.13 Mutagen Concentration Test - Ninth Protocol.

• Finished updating the wiki.

• Started approximately 30mL of E. coli B overnight.