Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Explist

From 2013.igem.org

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<font size="4"> '''9.7 CsCl Gradient''' </font>
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<font size="4"> '''10.16 T7 EM''' </font>
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This procedure purifies mutated T7 phage from the small phage team.
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This procedure details how we got EM images of T7 phage.
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Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.76CsClGradient|9.7 CsCl Gradient]]
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:-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/10.16T7EM| 10.16 T7 EM]]
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<font size="4"> '''9.9 10X  Phage Suspension Buffer Creation''' </font>
 
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We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients.
 
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:-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]]
 
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<font size="4"> '''9/11/13''' </font>
 
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- We ran another T7 phage purification procedure with new PSB.
 
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: -[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]]
 

Latest revision as of 21:04, 21 October 2013


Phage Purification August - September Notebook: September 1 - September 14 Experiment Listing



Phage Purification
March-April
May-June
July-August
September-October

10.16 T7 EM

This procedure details how we got EM images of T7 phage.

- 10.16 T7 EM





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