Team:KU Leuven/Project/E.coligy

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<div id="container">
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<div class="container">
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<!--LOGOS-->
<div class="row-fluid">
<div class="row-fluid">
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  <div class="span12 white">
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  <h3>Index</h3>
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  <div class="span4 icon white">
   <div class="row-fluid">
   <div class="row-fluid">
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   <div class="span4 greytext">
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   <div class="visible-desktop span3 bg-grey1">
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     <a href="#Methyl Salicylate"><h4>Methyl Salicylate</h4></a>
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     <a href="https://2013.igem.org/Team:KU_Leuven/Project/Aphid_Background">
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     <a href="#Effect on plants"><p>Plants</p></a>
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     <i class="livicon activeicon" data-name="bug" data-color="white"></i></div>
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    <a href="#Effect on aphids"><p>Aphids</p></a>
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  <div class="span7 icon-text">
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    <a href="#Effect on predators"><p>Predators</p></a>
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    <h3>Aphid Background</h3>
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    <a href="#Biobest and pc fruit insect experiments"><p>Insect experiments</p></a>
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    </a>
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    <p>Crashcourse in aphid biology</p>
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    <a href="#E-beta-farnesene"><h4>E-beta-farnesene</h4></a>
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     <a href="#Effect on aphids"><p>Aphids</p></a>
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  <div class="span4 icon white">
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    <a href="#Effect on predators"><p>Predators</p></a>
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  <div class="row-fluid">
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    <a href="#Biobest and pc fruit insect experiments"><p>Insect experiments</p></a>
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  <div class="visible-desktop span3 bg-grey1">
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     <a href="https://2013.igem.org/Team:KU_Leuven/Project/HoneydewSystem">
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    <i class="livicon activeicon" data-name="drop" data-color="white"></i></div>
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  <div class="span7 icon-text">
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    <h3>Honeydew System</h3> </a>
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    <p>Our BanAphids react to honeydew</p>
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  </div>
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     <a href="#Ecology Model"><h4>Ecology Model</h4></a>
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    <a href="#Ecology Model"><p>Ecology Model</p></a>
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  <div class="visible-desktop span3 bg-grey1">
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     <a href="https://2013.igem.org/Team:KU_Leuven/Project/StickerSystem">
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    <i class="livicon activeicon" data-name="refresh" data-color="white"></i>
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    </div>
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  <div class="span7 icon-text">
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    <h3>Sticker System</h3>
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    </a>
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    <p>BanAphid population oscillates!</p>
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  <a id="Methyl Salicylate"></a>
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  <h3>Methyl Salicylate</h3>
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     <h3><br/></h3>
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     <a href="https://2013.igem.org/Team:KU_Leuven/Project/E.coligy">
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    <img src="https://static.igem.org/mediawiki/2013/1/17/MeSA.gif"/>
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    <i class="livicon activeicon" data-name="leaf" data-color="white"></i>
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    <p>Figure 1ǀ Induced-resistance systems in plants</p>
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     <h3><i>E. coligy</i></h3>
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     <a id="Effect on plants"></a>
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     </a>
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    <h3>Plants</h3>
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    <p>You are here!</p>
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     <p align="justify">Plant resistance to biotrophic pathogens is classically thought to be mediated through SA signalling. Salicylic acid (SA), a phenolic phytohormone, is involved in many functions such as mediating in plant defence against pathogens. SA induces the production of pathogenesis-related (PR) proteins and is involved in the systemic acquired resistance (SAR), which is a "whole-plant" resistance response that occurs following an earlier localised exposure to a pathogen. SAR is analogous to the innate immune system found in animals. The resistance observed following induction of SAR is effective against a wide range of pathogens and the activation of SAR requires the accumulation of endogenous SA. SA modifications such as methylation and amino acid conjugation provide biological specificity in plant defence responses (Loake et al. 2007).
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Methyl salicylate (MeSA), a volatile ester, is normally absent in plants but is dramatically induced upon pathogen infection. It acts as a mobile or volatile inducer of SAR by carrying this ‘under attack’ signal to neighbouring plants, following hydrolysis by methyl esterase in it’s immediate surrounding. MeSA is synthesised by SA carboxyl methyltransferase (SAMT).
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    <a id="Effect on aphids"></a>
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  <a href="https://2013.igem.org/Team:KU_Leuven/Parts">
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     <h3>Aphids</h3>
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    <i class="livicon activeicon" data-name="gears" data-color="white"></i></div>
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    <p align="justify">Methyl salicylate (MeSA), a volatile ester, is normally absent in plants but is dramatically induced upon pathogen infection. It acts as a mobile or volatile inducer of SAR by carrying this ‘under attack’ signal to neighbouring plants, following hydrolysis by methyl esterase in it’s immediate surrounding. MeSA is synthesised by SA carboxyl methyltransferase (SAMT).</p>
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  <div class="span7 icon-text">
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     <h3>Parts</h3> </a>
 +
    <p>BioBrick 'm all!</p>
   </div>
   </div>
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  </div>
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  <div class="span4 greytext">
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</div>
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    <h3><br/></h3>
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     <img src="https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg" alt="Aphid colors"/>
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<div class="span4 icon white">
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     <p>Figure 2ǀ Red, yellow-green and green pea aphids.</p>
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  <div class="row-fluid">  
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    <div class="visible-desktop span3 bg-grey1">
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     <a href="https://2013.igem.org/Team:KU_Leuven/Project/DataPage">
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    <i class="livicon activeicon" data-name="info" data-color="white"></i>
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     </div>
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  <div class="span7 icon-text">
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    <h3>Data Page</h3> </a>
 +
    <p>All our achievements on one small page!</p>
   </div>
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   </div>
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    <img src="https://static.igem.org/mediawiki/2013/1/17/MeSA.gif"/>
 
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    <p>Figure 1ǀ Induced-resistance systems in plants</p>
 
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    <a id="Effect on predators"></a>
 
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    <h3>Predators</h3>
 
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    <p align="justify"> Methyl salicylate (MeSA)
 
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   <h3 class="bg-oscillator"><i>E. coligy</i></h3>
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  <h3>E-beta-farnesene</h3>
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</div>
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<div class="row-fluid">
<div class="row-fluid">
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  <div class="span12 white">
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  <p align = "justify">Our <B>BanAphids</B> produce <b>Methyl Salicylate (MeS) and E-β-farnesene (EBF)</b>, a phytohormone and a pheromone, respectively. This will offer the plants <b>a protection mechanism</b>. MeS will activate plant defence systems and attract natural aphid predators and parasitoids. Furthermore, EBF will repel aphids off the plant and, as a secondary effect, also attract natural aphid predators and parasitoids. MeS and EBF are naturally used in the communication between plants, predators and aphids. Consequentely, our <B>BanAphids</B> will blend into the ecosystem and enhance the communication between plants, predators and aphids.If you want to <b>expand your knowledge</b> about these natural occuring communication systems, you can read the <b><a href="https://2013.igem.org/Team:KU_Leuven/Project/Ecological/Background">background page</a></b>.<br/><br/>
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    <img src="https://static.igem.org/mediawiki/2013/1/17/MeSA.gif"/>
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-
    <p>Figure 1ǀ Induced-resistance systems in plants</p>
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Various experiments with both plants and insects were performed by us. In the <b><a href="https://2013.igem.org/Team:KU_Leuven/Project/Ecological/wetlab">wetlab page</a></b>. This validates that our <b>BanAphids</b> will work as intended and allows us to optimise our system.</br></br>
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We also modelled the impact of our BanAphids on the environment, which can be found in the <b>Modelling tab</b>, under the under <b><a href="https://2013.igem.org/Team:KU_Leuven/Project/Modelling/Ecosystem_Level">Ecosystem level</a></b>.The information obtained here is useful to plan field experiments and gives essential information to future costumers.
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    <a id="Effect on aphids"></a>
 
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    <h3>Aphids</h3>
 
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Methyl salicylate (MeSA), a volatile ester, is normally absent in plants but is dramatically induced upon pathogen infection. It acts as a mobile or volatile inducer of SAR by carrying this ‘under attack’ signal to neighbouring plants, following hydrolysis by methyl esterase in it’s immediate surrounding. MeSA is synthesised by SA carboxyl methyltransferase (SAMT).
 
</p>
</p>
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  <div class="span3 greytext">
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  <img src="https://static.igem.org/mediawiki/2013/0/09/Aphidinfestedrose.png"/>
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  <p><B>Aphid infested Rose plant</B> (Mauro Mandrioli)</p>
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  </div>
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  </div>
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<div class="row-fluid">
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     <a id="Effect on predators"></a>
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     <a href="https://2013.igem.org/Team:KU_Leuven/Project/Ecological/Background">
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    <h3>Predators</h3>
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     <i class="livicon activeicon" data-name="leaf" data-onparent="true" data-color="white"></i>
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     <p align="justify">Methyl salicylate (MeSA)
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     <h3>Background</h3> </a>
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     <img src="https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg" alt="Aphid colors"/>
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     <div class="row-fluid">
-
    <p>Figure 2ǀ Red, yellow-green and green pea aphids.</p>
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    <div class="span12">
 +
      <p align="justify">The effects of MeS and EBF on the ecosystem are tested in the <b><i>E. coligy</i> wetlab</b> section. If you want to know more about what is already known about these components you are invited to check out this <b>background</b> page. We also <a href="https://2013.igem.org/Team:KU_Leuven/Project/Modelling/Ecosystem_Level">modelled</a> the potential impact of our BanAphids on the ecosystem.</p>
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    </div>
   </div>
   </div>
   </div>
   </div>
  </div>
  </div>
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<!-- Insect experiments -->
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<!-- Wetlab -->
<div class="row-fluid">
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     <a href="https://2013.igem.org/Team:KU_Leuven/Project/Ecological/wetlab">
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    <img src="https://static.igem.org/mediawiki/2013/1/17/MeSA.gif"/>
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     <i class="livicon activeicon" data-name="lab" data-onparent="true" data-color="white"></i>
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     <p>Figure 1ǀ Induced-resistance systems in plants</p>
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     <a id=" Insect experiments"></a>
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     <h3>Wetlab</h3> </a>
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     <h3> Insect experiments</h3>
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     <div class="row-fluid">
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    <p align="justify"> Methyl salicylate (MeSA)
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      <p align="justify">We wanted to perform <b>behavioural experiments with aphids and aphid predators</b> such as ladybugs and the green lacewing. Therefore we presented our project to several companies: <a href="http://www.biobest.be"><b>Biobest</b></a>, a worldwide leader in biological pollination and sustainable crop management, and <a href="http://www.pcfruit.be"><b>pcfruit</b></a>, a company focussing on new crop protection methods. They were both very enthousiastic about our project and we formed a collaboration with Biobest and pc fruit to carry out our <b><i>E. coligy</i> experiments</b> at their site. On this page you will find more information about these experiments.</p>
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Latest revision as of 19:05, 28 October 2013

iGem

Secret garden

Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!


  • A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
  • For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
  • We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?

Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!

tree ladybugcartoon

Aphid Background

Crashcourse in aphid biology

Honeydew System

Our BanAphids react to honeydew

Sticker System

BanAphid population oscillates!

E. coligy

You are here!

Parts

BioBrick 'm all!

Data Page

All our achievements on one small page!

Our BanAphids produce Methyl Salicylate (MeS) and E-β-farnesene (EBF), a phytohormone and a pheromone, respectively. This will offer the plants a protection mechanism. MeS will activate plant defence systems and attract natural aphid predators and parasitoids. Furthermore, EBF will repel aphids off the plant and, as a secondary effect, also attract natural aphid predators and parasitoids. MeS and EBF are naturally used in the communication between plants, predators and aphids. Consequentely, our BanAphids will blend into the ecosystem and enhance the communication between plants, predators and aphids.If you want to expand your knowledge about these natural occuring communication systems, you can read the background page.

Various experiments with both plants and insects were performed by us. In the wetlab page. This validates that our BanAphids will work as intended and allows us to optimise our system.

We also modelled the impact of our BanAphids on the environment, which can be found in the Modelling tab, under the under Ecosystem level.The information obtained here is useful to plan field experiments and gives essential information to future costumers.

Aphid infested Rose plant (Mauro Mandrioli)

Background

The effects of MeS and EBF on the ecosystem are tested in the E. coligy wetlab section. If you want to know more about what is already known about these components you are invited to check out this background page. We also modelled the potential impact of our BanAphids on the ecosystem.

Wetlab

We wanted to perform behavioural experiments with aphids and aphid predators such as ladybugs and the green lacewing. Therefore we presented our project to several companies: Biobest, a worldwide leader in biological pollination and sustainable crop management, and pcfruit, a company focussing on new crop protection methods. They were both very enthousiastic about our project and we formed a collaboration with Biobest and pc fruit to carry out our E. coligy experiments at their site. On this page you will find more information about these experiments.

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