Team:EPF Lausanne/Protocols

From 2013.igem.org

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[https://static.igem.org/mediawiki/2013/6/61/Team-EPF-Lausanne_Immunofl_staining_of_cellsurface_proteins.pdf Immunifluorescent staining of Cell Surface Proteins] <BR>
[https://static.igem.org/mediawiki/2013/6/61/Team-EPF-Lausanne_Immunofl_staining_of_cellsurface_proteins.pdf Immunifluorescent staining of Cell Surface Proteins] <BR>
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'''Microfluidics experiment'''
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'''Microfluidics experiment''' <BR>
[https://static.igem.org/mediawiki/2013/7/7a/Team-EPF-Lausanne_MITOMI_protocol.pdf MITOMI] <BR>
[https://static.igem.org/mediawiki/2013/7/7a/Team-EPF-Lausanne_MITOMI_protocol.pdf MITOMI] <BR>

Revision as of 19:16, 28 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Protocols

PCR
OD Measurements
Measuring Fluorescence on a Plate Reader
Transforming Cells
Dialysis
ELISA-like Assay
Making Nanoparticles
Making Competent Cells
Gibson Assembly
Western Blot
Biotin Staining of Streptavidin
Direct Staining of cell surface Proteins
Immunifluorescent staining of Cell Surface Proteins

Microfluidics experiment
MITOMI

Datasheets

Below are listed the protocols we took directly from the datasheets/manufacturer instructions:

[http://www.piercenet.com/instructions/2160236.pdf Biotinylation]

[http://www.piercenet.com/instructions/2160383.pdf Chemically attaching streptavidin to bacterial surface] (using bacteria's outer glycoproteins)

[http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/m9070pis.pdf MMP2 activation]

Wiki Links

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