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Team:Tianjin/Project/Futurework
From 2013.igem.org
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In this summer, we construct a new alkane sensing system AlkR-PalkM, all the achievements are based on this system. | In this summer, we construct a new alkane sensing system AlkR-PalkM, all the achievements are based on this system. | ||
| - | #Based on the system of AlkR-PalkM , we use this to build an Alk-Sensor based on the system of AlkR-PalkM which can detect the alkane which is produced by engineered E. coli. | + | #1.Based on the system of AlkR-PalkM , we use this to build an Alk-Sensor based on the system of AlkR-PalkM which can detect the alkane which is produced by engineered E. coli. |
| - | #We characterized the Alk-Sensor of different aspects. | + | #2.We characterized the Alk-Sensor of different aspects. |
| - | #Based on Alk-Sensor, we constructed Alk-Selector for direct evolution | + | #3.Based on Alk-Sensor, we constructed Alk-Selector for direct evolution |
| - | #We used Alk-Selector to optimize the function of NPDC. | + | #4.We used Alk-Selector to optimize the function of NPDC. |
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Revision as of 20:06, 28 October 2013
Summary:
In this summer, we construct a new alkane sensing system AlkR-PalkM, all the achievements are based on this system.
- 1.Based on the system of AlkR-PalkM , we use this to build an Alk-Sensor based on the system of AlkR-PalkM which can detect the alkane which is produced by engineered E. coli.
- 2.We characterized the Alk-Sensor of different aspects.
- 3.Based on Alk-Sensor, we constructed Alk-Selector for direct evolution
- 4.We used Alk-Selector to optimize the function of NPDC.
Future work:
All parts are also not perfect, needed to be optimized.
- It may be necessary to mutate PalkM to reduce the leakage of the system.
- Different reporter needed to be tried to get different relationship between input and output.
- Mutate AlkR may promote the interaction between AlkR and Alkane, and improve the function of AlkR-PalkM system.
- Addition of gene related to biosufactant biosynthesis may increasing the sensing range of the alkane
- For biofuel synthetic, Alk-Selector provide the more opportunities to engineered the strain. Many method related to build a library can be used in this field with the help of Alk-Selector.
