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Team:Tianjin/Project/Futurework
From 2013.igem.org
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All parts are also not perfect, needed to be optimized. | All parts are also not perfect, needed to be optimized. | ||
| - | #It may be necessary to mutate PalkM to reduce the leakage of the system. | + | #1.It may be necessary to mutate PalkM to reduce the leakage of the system. |
| - | #Different reporter needed to be tried to get different relationship between input and output. | + | #2.Different reporter needed to be tried to get different relationship between input and output. |
| - | #Mutate AlkR may promote the interaction between AlkR and Alkane, and improve the function of AlkR-PalkM system. | + | #3.Mutate AlkR may promote the interaction between AlkR and Alkane, and improve the function of AlkR-PalkM system. |
| - | #Addition of gene related to biosufactant biosynthesis may increasing the sensing range of the alkane | + | #4.Addition of gene related to biosufactant biosynthesis may increasing the sensing range of the alkane |
| - | #For biofuel synthetic, Alk-Selector provide the more opportunities to engineered the strain. Many method related to build a library can be used in this field with the help of Alk-Selector. | + | #5.For biofuel synthetic, Alk-Selector provide the more opportunities to engineered the strain. Many method related to build a library can be used in this field with the help of Alk-Selector. |
Revision as of 20:07, 28 October 2013
Summary:
In this summer, we construct a new alkane sensing system AlkR-PalkM, all the achievements are based on this system.
- 1.Based on the system of AlkR-PalkM , we use this to build an Alk-Sensor based on the system of AlkR-PalkM which can detect the alkane which is produced by engineered E. coli.
- 2.We characterized the Alk-Sensor of different aspects.
- 3.Based on Alk-Sensor, we constructed Alk-Selector for direct evolution
- 4.We used Alk-Selector to optimize the function of NPDC.
Future work:
All parts are also not perfect, needed to be optimized.
- 1.It may be necessary to mutate PalkM to reduce the leakage of the system.
- 2.Different reporter needed to be tried to get different relationship between input and output.
- 3.Mutate AlkR may promote the interaction between AlkR and Alkane, and improve the function of AlkR-PalkM system.
- 4.Addition of gene related to biosufactant biosynthesis may increasing the sensing range of the alkane
- 5.For biofuel synthetic, Alk-Selector provide the more opportunities to engineered the strain. Many method related to build a library can be used in this field with the help of Alk-Selector.
