Team:EPF Lausanne/Protocols

From 2013.igem.org

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(Protocols)
 
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[https://static.igem.org/mediawiki/2013/4/45/Team-EPF-Lausanne_PCR.pdf PCR] <BR>
[https://static.igem.org/mediawiki/2013/4/45/Team-EPF-Lausanne_PCR.pdf PCR] <BR>
[https://static.igem.org/mediawiki/2013/6/6f/Team-EPF-Lausanne_OD_Measurements.pdf OD Measurements]<BR>
[https://static.igem.org/mediawiki/2013/6/6f/Team-EPF-Lausanne_OD_Measurements.pdf OD Measurements]<BR>
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[https://static.igem.org/mediawiki/2013/3/34/Team-EPF-Lausanne_PlateReader_Measurements.pdf PlateReader Measurements] <BR>
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[https://static.igem.org/mediawiki/2013/3/34/Team-EPF-Lausanne_PlateReader_Measurements.pdf Measuring Fluorescence on a Plate Reader] <BR>
[https://static.igem.org/mediawiki/2013/1/15/Team-EPF-Lausanne_Transforming_Cell.pdf Transforming Cells]<BR>
[https://static.igem.org/mediawiki/2013/1/15/Team-EPF-Lausanne_Transforming_Cell.pdf Transforming Cells]<BR>
[https://static.igem.org/mediawiki/2013/f/fa/Team-EPF-Lausanne_Protocol_dialysis.pdf Dialysis ] <BR>
[https://static.igem.org/mediawiki/2013/f/fa/Team-EPF-Lausanne_Protocol_dialysis.pdf Dialysis ] <BR>
[https://static.igem.org/mediawiki/2013/1/17/Team-EPF-Lausanne_ELISA-like_assay.pdf ELISA-like Assay] <BR>
[https://static.igem.org/mediawiki/2013/1/17/Team-EPF-Lausanne_ELISA-like_assay.pdf ELISA-like Assay] <BR>
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[https://static.igem.org/mediawiki/2013/d/df/Team-EPF-Lausanne_Making_Nanoparticles.pdf Making Nanoparticles] <BR>
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[https://static.igem.org/mediawiki/2013/6/6e/Team-EPF-Lausanne_Making_CompetentCell.pdf Making Competent Cells] <BR>
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[https://static.igem.org/mediawiki/2013/d/de/Team-EPF-Lausanne_Gibson_Assembly.pdf Gibson Assembly] <BR>
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[https://static.igem.org/mediawiki/2013/4/4a/Team-EPF-Lausanne_Western_Blot.pdf Western Blot] <BR>
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[https://static.igem.org/mediawiki/2013/b/bd/Team-EPF-Lausanne_Biotin_Staining_of_Streptavidin.pdf Biotin Staining of Streptavidin]<BR>
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[https://static.igem.org/mediawiki/2013/a/a4/Team-EPF-Lausanne_Staining_of_Cell_Surface_Protein.pdf Direct Staining of cell surface Proteins] <BR>
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[https://static.igem.org/mediawiki/2013/6/61/Team-EPF-Lausanne_Immunofl_staining_of_cellsurface_proteins.pdf Immunifluorescent staining of Cell Surface Proteins] <BR>
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'''Microfluidics experiment''' <BR>
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[https://static.igem.org/mediawiki/2013/7/7a/Team-EPF-Lausanne_MITOMI_protocol.pdf MITOMI] <BR>
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[https://static.igem.org/mediawiki/2013/e/ed/Team-EPF-Lausanne_pdms2.pdf PDMS two layer device fabrication] <BR>
===Datasheets===
===Datasheets===
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[http://www.piercenet.com/instructions/2160383.pdf Chemically attaching streptavidin to bacterial surface] (using bacteria's outer glycoproteins)
[http://www.piercenet.com/instructions/2160383.pdf Chemically attaching streptavidin to bacterial surface] (using bacteria's outer glycoproteins)
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[http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/m9070pis.pdf MMP2 activation]
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Latest revision as of 20:28, 28 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Protocols

PCR
OD Measurements
Measuring Fluorescence on a Plate Reader
Transforming Cells
Dialysis
ELISA-like Assay
Making Nanoparticles
Making Competent Cells
Gibson Assembly
Western Blot
Biotin Staining of Streptavidin
Direct Staining of cell surface Proteins
Immunifluorescent staining of Cell Surface Proteins

Microfluidics experiment
MITOMI
PDMS two layer device fabrication

Datasheets

Below are listed the protocols we took directly from the datasheets/manufacturer instructions:

[http://www.piercenet.com/instructions/2160236.pdf Biotinylation]

[http://www.piercenet.com/instructions/2160383.pdf Chemically attaching streptavidin to bacterial surface] (using bacteria's outer glycoproteins)

[http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/m9070pis.pdf MMP2 activation]