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Team:Tianjin/Project/Futurework
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| - | <a href="https://2013.igem.org/Team:Tianjin/Project/ | + | <a href="https://2013.igem.org/Team:Tianjin/Project/Alk-Selector&DirectEvolution">Alk-Selector And Direct Evolution</a> |
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Revision as of 20:47, 28 October 2013
Summary
In this summer, we construct a new alkane sensing system AlkR-PalkM, all the achievements are based on this system.
- 1.Based on the system of AlkR-PalkM , we use this to build an Alk-Sensor based on the system of AlkR-PalkM which can detect the alkane which is produced by engineered E. coli.
- 2.We characterized the Alk-Sensor of different aspects.
- 3.Based on Alk-Sensor, we constructed Alk-Selector for direct evolution
- 4.We used Alk-Selector to optimize the function of NPDC.
Future work
All parts are also not perfect, needed to be optimized.
- 1.It may be necessary to mutate PalkM to reduce the leakage of the system.
- 2.Different reporter needed to be tried to get different relationship between input and output.
- 3.Mutate AlkR may promote the interaction between AlkR and Alkane, and improve the function of AlkR-PalkM system.
- 4.Addition of gene related to biosufactant biosynthesis may increasing the sensing range of the alkane
- 5.For biofuel synthetic, Alk-Selector provide the more opportunities to engineered the strain. Many method related to build a library can be used in this field with the help of Alk-Selector.
