Team:EPF Lausanne/Calendar/28 October 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display</font>  
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<font size = "4"> Cell Surface Display</font>
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<BR>''CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)'' <BR>
<BR>''CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)'' <BR>
Repeating the microscopy
Repeating the microscopy
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<br>- samples 1 for IYS and 3 for ISY were chosed and prepared as the day before based on our protocols
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<br>- Samples 1 for IYS and 3 for ISY were chosed and prepared as the day before based on our protocols
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<br>- different aliquots of these samples were incubated separatly with a FITC conjugated anti-streptavidin antibody; a Phicoerythrin conjugated anti-GFP antibody; biotynilated nanoparticles and at the end with biotin conjugated anti-GFP antibody (revealed with avidin dylight (red)).
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<br>- Different aliquots of these samples were incubated separatly with a FITC conjugated anti-streptavidin antibody; a Phicoerythrin conjugated anti-GFP antibody; biotynilated nanoparticles and at the end with biotin conjugated anti-GFP antibody (revealed with avidin dylight (red)).
<br>- Some images of this microscopy are on our wiki page. It shows that the construct is expressed and that YFP is likely to be on the outer membrane.
<br>- Some images of this microscopy are on our wiki page. It shows that the construct is expressed and that YFP is likely to be on the outer membrane.

Revision as of 23:14, 28 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display


CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)

Repeating the microscopy
- Samples 1 for IYS and 3 for ISY were chosed and prepared as the day before based on our protocols
- Different aliquots of these samples were incubated separatly with a FITC conjugated anti-streptavidin antibody; a Phicoerythrin conjugated anti-GFP antibody; biotynilated nanoparticles and at the end with biotin conjugated anti-GFP antibody (revealed with avidin dylight (red)).
- Some images of this microscopy are on our wiki page. It shows that the construct is expressed and that YFP is likely to be on the outer membrane.



Nanoparticles

Microfluidic
- Repeat microfluidics experiment for nanoparticles digestion with MMP2

Wiki Links

Wiki Links

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