Team:BGU Israel

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                          <h6 style="text-align:center"><b>Follow the story of BGU_Israel: Making the dream of Synthetic Biology a reality today!</b><hr/></h6></br>
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<img src="https://static.igem.org/mediawiki/2013/d/db/BGU_01.jpg" title= "<slide-text>About 60 years ago, David Ben-Gurion, Israel's first prime minister, had a vision of developing the Negev desert.</br> <i>&quot;It is in the Negev that the creativity and pioneer vigor of Israel will be tested&quot;</i></slide-text>" alt="" />
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<img src="https://static.igem.org/mediawiki/2013/7/74/BGU_02.jpg" title="<slide-text>In 1969, his dream became a reality. Ben-Gurion Univeristy (BGU) was established in the Negev desert.</slide-text>" alt="" />
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                                <img src="https://static.igem.org/mediawiki/2013/4/4f/BGU_03.jpg" title="<slide-text>BGU's exellence in engineering, research and science is a fullfilment of Ben-Gurion's vision. </br> As Ben-Gurion has stated:<i>&quot; It is incumbent upon our researchers to focus on new areas of research &quot;</i></slide-text>  " alt="" />
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                              <img src="https://static.igem.org/mediawiki/2013/d/d7/BGU_04.jpg" title="<slide-text>In 2013, the pioneer iGEM team BGU_Israel is established. </br> Follow our journey from start to finish.</slide-text> " alt="" />
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                  </br>           
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                            </br>
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                                <p>Our story began at the middle of the third moon cycle, 2013, in the mysterious desert city of the seven wells - Beer sheva. </br>
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                                It all started when two wandering friends heard about the epic journey of iGEM, immediately they knew they want to embark on this adventure.
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                                </br>A band of 12 undergrad-adventurers from 10 different disciplines was then assembled for this pioneering quest.  Against all odds they managed to learn about iGEM and introduce it to the Uni.
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                                </br>Along with public, recruit the instructors, raise the funds create the project and get to the finals!  In some encounters it is hinted that they even had fun!
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                                </br>
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                                We invite you to join our journey to make the synthetic biology dream come true today.
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                              </p>
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                            <div class="time1">
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                                  <div class="time">Environmental Problem</div>
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                                  <span class="corner-left"></span>
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                                  <a href="/Team:BGU_Israel/SafetyTheProblem"><img src="https://static.igem.org/mediawiki/2013/0/09/BGU_GMO3.jpg"  /></a>
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                                  <p>To embark upon the quest, we thought of dozens of crazy ideas but in vain. Even if the idea was truly novel and practical we could not find a way to make it work because we couldn't see how our Magical Genetically-Engineered-Machines could be contained once introduced to the environment.
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                                  </br>This safety-environmental concern possessed our minds day and night. Alas, we could find neither tools nor weapons to relieve our concerns.
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                                  </br>
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                                  Read about <a href="/Team:BGU_Israel/SafetyTheProblem">the environmental problem</a>
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                                  </p>
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                                </div>
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                            </div>
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                                    <div class="time">Survey</div>
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                                    <span class="corner-right"></span>
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                                    <a href="/Team:BGU_Israel/Survey"><img src="https://static.igem.org/mediawiki/2013/5/58/BGU_survey22.png"  /></a>
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                                    <p>
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                                    Our first step was to undertake a massive public opinion survey to learn about the current state of public opinion, and hopefully understand the roots of opposition to synthetic biology.
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                                    </br>We realized that the public concerns stand in the way of new Synthetic Biology products. We also realized that these concerns could be relieved if a self-destructive mechanism was available.
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                                    </br>
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                                    Read the full <a href="/Team:BGU_Israel/Survey">survey</a> analysis.
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                                    </p>
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                                </div>
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                            </div>
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                                  <div class="time">Motivation</div>
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                                  <span class="corner-left"></span>
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                                  <a href="/Team:BGU_Israel/Problem"><img src="https://static.igem.org/mediawiki/2013/7/78/BGU_synbio.jpg"  /></a>
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                                  <p>Taking into consideration the public concerns and the environmental hazards, we had a breakthrough! We will develop a self-destruct mechanism that will allow for safe and controlled usage of any GMO.
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                                  </br>This could be a step in making the Synthetic Biology Dream become a reality.
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                                  </br>
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                                  Read about our project's <a href="/Team:BGU_Israel/Problem">motivation</a>.
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                                  </p>
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                                </div>
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                                  <div class="time">Our Solution</div>
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                                  <a href="/Team:BGU_Israel/Solution"><img src="https://static.igem.org/mediawiki/2013/6/67/BGU_diagram.png"  /></a>
 +
                                  <p>Inspired by this idea, we sketched two different generic systems to be inserted into GEMs under the strict guidelines that the systems have to be both Programmable in the lab and Autonomous of outside environment.
 +
                                  </br>We labeled our systems as Programmable Autonomous Self Elimination (P.A.S.E) 1 and 2. 
 +
                                  </br>
 +
                                  Read about <a href="/Team:BGU_Israel/Solution">our solution</a>.
 +
                                  </p>
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                                </div>
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                                  <div class="time">Get a Glimpse</div>
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                                  <p>Watch a video that explains the high-level concepts behind our project.  
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                                  <div class="time">Experiments & Results</div>
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                                  <p>At this point you must be eager to know how did we make our idea work. We spent day and night in the wet lab, developing and experimenting.  
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                                  </br>Join us and follow the entire process in our <a href="/Team:BGU_Israel/Experiments">experiments & results</a>. 
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                                  <div class="time">Modelling</div>
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                                  <p>The team's mathematicians worked hard to create a stochastic model that could     
 +
                                  predict if and to what extent the system could be useful and fail proof.   
 +
                                  </br>
 +
                                  Read more about <a href="/Team:BGU_Israel/ModelingOverView.html">modelling</a>.
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                                  <div class="time">Human Practice</div>
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                                  <a href="/Team:BGU_Israel/HPOverview"><img src="https://static.igem.org/mediawiki/2013/f/f9/BGU_topic_outreach.png"  /></a>
 +
                                  <p>After creating our novel solution, we were excited to go back to the public and spread the word. We embarked on a massive outreach campaign to encourage discourse with the public about synthetic biology, in order to dispel misconceptions and foster debate. Our outreach campaign was aimed at reaching as many people from different communities and age groups as possible. We wanted to burst out of the invisible walls separating the academy from the general public and trigger discussions in a variety of platforms, making the subject accessible to all.
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                                  </br>
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                                  Follow our <a href="/Team:BGU_Israel/HPOverview">Human Practice</a> activities.
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                                  <div class="time">The Team</div>
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                                  <p>Thank you for following our Journey. Want to know the people behind the project?
 +
                                  </br>
 +
                                  Come and meet <a href="/Team:BGU_Israel/Team">the team</a>.
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                                  </p>
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                                  <h6></br>Our achievements in the European Jamboree: </h6></br>
 +
                                  <ul>
 +
                                  <li class="list-v"> &nbsp;&nbsp;Silver Medal</li>
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                                  <li class="list-v"> &nbsp;&nbsp;Best Movie</li>
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                                  <li class="list-v"> &nbsp;&nbsp;Best Presentation</li>
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                                  <li class="list-v"> &nbsp;&nbsp;Advance to World Championship!</li>
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                                  </ul>
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                                  </br>
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<li class="current_page_item"><a href="index.html">Representing Ben-Gurion University from Israel in iGEM 2013</a></li>
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<span><strong> P.A.S.E - Programmable Autonomous Self Elimination</strong></span>
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<h2><strong>Project Description</strong></h2>
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<p>The processes of bioremediation, biosensors and bio-medicine often require the release of genetically modified organisms (GMO's) to the environment. After being released, these GMO's are no longer under direct control, therefore posing potential threats such as substituting the natural bacterial population, horizontal gene transfer, and lastly, creating public concern and opposition to the field of genetic engineering.<br/><br/>
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In order to overcome these issues, we are designing a genetic circuit that limits the lifetime of a bacterial population after it is released into the environment. Our ultimate goal is to allow the end user to program a GMO population to survive in the environment until it has completed its task, after which the entire population will disappear. Crucially, our mechanism will function independently, without any external intervention. <br/><br/>
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We are approaching this goal from two angles: <strong>“P.A.S.E 1” </strong>is based on the dilution of a vital component through cell division, while <strong> “P.A.S.E 2" </strong> is based on the lifetime of an essential protein. The two methods utilize novel mechanisms such as a recombination cassette, the innovative use of an unnatural amino acid (UAA) in a biologic gate, and more. We also intend to introduce new biobricks that will be useful for a wide range of purposes, including bacterial genome incorporation parts and UAA incorporation machinery. This project will be the first modular, independent and generic bacterial control system. <br/><br/>
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We want our construct to pave the way to a safe synthetic revolution: one that will allow developers to innovate and create freely without arousing public opposition or harming the environment. <br/><br/>
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<strong>more to come from BGU_Israel...</strong>
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Latest revision as of 23:34, 28 October 2013

BGU_Israel



Follow the story of BGU_Israel: Making the dream of Synthetic Biology a reality today!



    Our story began at the middle of the third moon cycle, 2013, in the mysterious desert city of the seven wells - Beer sheva.
    It all started when two wandering friends heard about the epic journey of iGEM, immediately they knew they want to embark on this adventure.
    A band of 12 undergrad-adventurers from 10 different disciplines was then assembled for this pioneering quest. Against all odds they managed to learn about iGEM and introduce it to the Uni.
    Along with public, recruit the instructors, raise the funds create the project and get to the finals! In some encounters it is hinted that they even had fun!
    We invite you to join our journey to make the synthetic biology dream come true today.

    Environmental Problem

    To embark upon the quest, we thought of dozens of crazy ideas but in vain. Even if the idea was truly novel and practical we could not find a way to make it work because we couldn't see how our Magical Genetically-Engineered-Machines could be contained once introduced to the environment.
    This safety-environmental concern possessed our minds day and night. Alas, we could find neither tools nor weapons to relieve our concerns.
    Read about the environmental problem

    Survey

    Our first step was to undertake a massive public opinion survey to learn about the current state of public opinion, and hopefully understand the roots of opposition to synthetic biology.
    We realized that the public concerns stand in the way of new Synthetic Biology products. We also realized that these concerns could be relieved if a self-destructive mechanism was available.
    Read the full survey analysis.

    Motivation

    Taking into consideration the public concerns and the environmental hazards, we had a breakthrough! We will develop a self-destruct mechanism that will allow for safe and controlled usage of any GMO.
    This could be a step in making the Synthetic Biology Dream become a reality.
    Read about our project's motivation.

    Our Solution

    Inspired by this idea, we sketched two different generic systems to be inserted into GEMs under the strict guidelines that the systems have to be both Programmable in the lab and Autonomous of outside environment.
    We labeled our systems as Programmable Autonomous Self Elimination (P.A.S.E) 1 and 2.
    Read about our solution.

    Get a Glimpse

    Watch a video that explains the high-level concepts behind our project.

    Experiments & Results

    At this point you must be eager to know how did we make our idea work. We spent day and night in the wet lab, developing and experimenting.
    Join us and follow the entire process in our experiments & results.

    Modelling

    The team's mathematicians worked hard to create a stochastic model that could predict if and to what extent the system could be useful and fail proof.
    Read more about modelling.

    Human Practice

    After creating our novel solution, we were excited to go back to the public and spread the word. We embarked on a massive outreach campaign to encourage discourse with the public about synthetic biology, in order to dispel misconceptions and foster debate. Our outreach campaign was aimed at reaching as many people from different communities and age groups as possible. We wanted to burst out of the invisible walls separating the academy from the general public and trigger discussions in a variety of platforms, making the subject accessible to all.
    Follow our Human Practice activities.

    The Team

    Thank you for following our Journey. Want to know the people behind the project?
    Come and meet the team.

    European Jamboree

    Our achievements in the European Jamboree:

    •   Silver Medal
    •   Best Movie
    •   Best Presentation
    •   Advance to World Championship!

Retrieved from "http://2013.igem.org/Team:BGU_Israel"