Team:Tianjin/Data

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                 <a href="https://2013.igem.org/Team:Tianjin/Human_Practice/NewTeamsGuidance">New Teams Guidance</a>
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                        <a href="">High School Inspiration</a>
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                 <a href="https://2013.igem.org/Team:Tianjin/Human_Practice/iGEMLeading-in">iGEM Leading-in</a>
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                        <a href="">iGEM Handbook</a>
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                        <a href="">The 1st Model iGEM</a>
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                        <a href="">Tsinghua University</a>
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                        <a href="">Ocean University of China</a>
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                        <a href=""> BIT-China</a>
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                        <a href="">PetroChina Dagang Petrochemical Company</a>
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                        <a href=""><li>Communicate with Research Institute</li></a>
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<div class="main">
<div class="main">
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=BioBrick=
 +
----
 +
 +
==Favorite Tianjin 2013 iGEM Team Parts==
 +
 +
<html>
 +
<style type="text/css">
 +
.table1{font-family:Arial, Helvetica, sans-serif;width:100%;border-collapse:collapse;}
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.table1 td, .table1 th{font-size:14px;border:1px solid #ccc;padding:5px 10px 5px 10px;width:150px;}
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.table1 th {font-size:14px;text-align:left;padding-top:5px;padding-bottom:4px;background-color:#ccc;color:#ffffff;}
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.table1 tr.alt td {color:#000000;background-color: #fafafa;}
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</style>
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 +
<table class="table1">
 +
<tr>
 +
<th>Name</th>
 +
<th>Type</th>
 +
<th>Description</th>
 +
<th>Designer</th>
 +
<th>Length</th>
 +
</tr>
 +
 +
<tr>
 +
<td> <a href="http://parts.igem.org/Part:BBa_K1020005" target="_blank">Bba_K1020005</a></td>
 +
<td>Composite</td>
 +
<td> Alkane Marker--Device for detection of various alkanes</td>
 +
<td>Boxuan Zeng<br/>Yue Liu </td>
 +
<td> 2146bp</td>
 +
</tr>
 +
 +
<tr>
 +
<td><a href="http://parts.igem.org/Part:BBa_K1020012" target="_blank">Bba_K1020012</a> </td>
 +
<td> Composite</td>
 +
<td> Alkane Selector for intracellular alkane production</td>
 +
<td> Boxuan Zeng<br/>Yue Liu </td>
 +
<td>2662bp </td>
 +
</tr>
 +
 +
</table>
 +
</html>
 +
 +
 +
==Tianjin 2013 iGEM Team Parts Sandbox==
 +
 +
 +
<html>
 +
 +
<table class="table1">
 +
<tr>
 +
<th>Name</th>
 +
<th>Type</th>
 +
<th>Description</th>
 +
<th>Designer</th>
 +
<th>Length</th>
 +
</tr>
 +
 +
<tr>
 +
<td><a href="http://parts.igem.org/Part:BBa_K1020001" target="_blank">Bba_K1020001</a></td>
 +
<td>Reporter</td>
 +
<td>PalkM promoter with reporter gene</td>
 +
<td>Boxuan Zeng<br/>Yue Liu</td>
 +
<td>1013bp</td>
 +
</tr>
 +
 +
<tr class="alt">
 +
<td><a href="http://parts.igem.org/Part:BBa_K1020002" target="_blank">Bba_K1020002</a></td>
 +
<td>Coding</td>
 +
<td>alkane regulatory protein ALKR</td>
 +
<td>Boxuan Zeng</td>
 +
<td>927bp </td>
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</tr>
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 +
<tr>
 +
<td><a href="http://parts.igem.org/Part:BBa_K1020003" target="_blank">Bba_K1020003 </a></td>
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<td> Reporter</td>
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<td> AlkR -PSB1C3-Low constitutive</td>
 +
<td>Yue Liu </td>
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<td> 1125bp</td>
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</tr>
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 +
<tr class="alt">
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<td><a href="http://parts.igem.org/Part:BBa_K1020004" target="_blank">Bba_K1020004 </a></td>
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<td> Composite</td>
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<td> Biosensor for alkane detection</td>
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<td> Yue Liu<br/>Su Wang</td>
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<td> 2009bp</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K1020006" target="_blank"> Bba_K1020006</a></td>
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<td>Coding </td>
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<td>YbhGFSR </td>
 +
<td>Boxuan Zeng<br/>Yang Wu  </td>
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<td> 4971bp</td>
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</tr>
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 +
<tr class="alt">
 +
<td><a href="http://parts.igem.org/Part:BBa_K1020007" target="_blank">Bba_K1020007 </a></td>
 +
<td> Composite</td>
 +
<td>AlkR -PSB1C3-High constitutive </td>
 +
<td>Yue Liu </td>
 +
<td>1125bp </td>
 +
</tr>
 +
 +
<tr>
 +
<td><a href="http://parts.igem.org/Part:BBa_K1020008" target="_blank">Bba_K1020008 </a></td>
 +
<td> Composite</td>
 +
<td>alkane producing module </td>
 +
<td> Boxuan Zeng<br/>Yue Liu</td>
 +
<td>1809bp </td>
 +
</tr>
 +
 +
<tr class="alt">
 +
<td><a href="http://parts.igem.org/Part:BBa_K1020009" target="_blank">Bba_K1020009</a> </td>
 +
<td>Composite </td>
 +
<td> YbhGFSR operon</td>
 +
<td> Boxuan Zeng</td>
 +
<td>5034bp </td>
 +
</tr>
 +
 +
<tr>
 +
<td><a href="http://parts.igem.org/Part:BBa_K1020010" target="_blank"> Bba_K1020010</a></td>
 +
<td>Coding </td>
 +
<td>TetA gene under the control of PalkM </td>
 +
<td> Boxuan Zeng<br/>Shoujie Sun</td>
 +
<td> 1529bp</td>
 +
</tr>
 +
 +
<tr class="alt">
 +
<td><a href="http://parts.igem.org/Part:BBa_K1020011" target="_blank"> Bba_K1020011</a></td>
 +
<td> Composite</td>
 +
<td>High expressed Alkane Selector for intracellular alkane production </td>
 +
<td>Boxuan Zeng<br/>Qingyi Deng </td>
 +
<td>2662bp </td>
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</tr>
 +
 +
 +
</table>
 +
 +
<a name="anchor-data" id="anchor-data"></a>
 +
</html>
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 +
=Others=
 +
----
 +
'''Results for Agarose Gel Electrophoresis:'''
 +
 +
[[File:Tju-gel-1.1.1.jpg|center|330px|gel 1]]
 +
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<html>
 +
<div style="text-align:center;">
 +
<b>Figure 1.</b>&nbsp;AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT</div>
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</html>
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 +
 +
[[File:Tju-gel-1.1.2.jpg|center|320px|gel 2]]
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<html>
 +
<div style="text-align:center;">
 +
<b>Figure 2.</b>&nbsp;AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT</div>
 +
</html>
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 +
[[File:Tju-gel-1.1.3.jpg|center|330px|gel 3]]
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<html>
 +
<div style="text-align:center;">
 +
<b>Figure 3.</b>&nbsp;J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT</div>
 +
 +
<a name="anchor-n-02" id="anchor-n-02"></a>
 +
<br/>
 +
</html>
 +
 +
[[File:Tju-gel-1.2.1.jpg|center|500px|gel 4]]
 +
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 4.</b>&nbsp;J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3</div>
 +
</html>
 +
 +
 +
[[File:Tju-gel-1.2.2.jpg|center|460px|gel 5]]
 +
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 5.</b>&nbsp;J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3</div>
 +
 +
 +
<a name="anchor-n-03" id="anchor-n-03"></a>
 +
<br/>
 +
</html>
 +
 +
[[File:Tju-gel-1.3.jpg|center|330px|gel 6]]
 +
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 6.</b>&nbsp;J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3</div>
 +
<BR/>
 +
</HTML>
 +
 +
'''Characterization for our construction:'''
 +
 +
 +
<HTML>
 +
<img src="https://static.igem.org/mediawiki/2013/3/3e/TJU-PROJECT-C3.jpg"/>
 +
 +
 +
 +
<div style="text-align:center;">
 +
<b>Figure 7.</b>&nbsp;The visible color change of the strain cultured in 3ML M9 medium with different induced for 12 hours.</div>
 +
 +
 +
 +
<DIV ALIGN="CENTER"><img src="https://static.igem.org/mediawiki/2013/f/fe/QQ图片20131029093339.jpg" width="400px"/></DIV>
 +
 +
 +
<div style="text-align:center;">
 +
<b>Figure 8.</b>&nbsp;Strain one contains alkane producer part(left),another contains none-producer part (right) .</div>

Latest revision as of 01:45, 29 October 2013

Contents

BioBrick

Favorite Tianjin 2013 iGEM Team Parts

Name Type Description Designer Length
Bba_K1020005 Composite Alkane Marker--Device for detection of various alkanes Boxuan Zeng
Yue Liu 		2146bp
Bba_K1020012 Composite Alkane Selector for intracellular alkane production Boxuan Zeng
Yue Liu 		2662bp


Tianjin 2013 iGEM Team Parts Sandbox

Name Type Description Designer Length
Bba_K1020001 Reporter PalkM promoter with reporter gene Boxuan Zeng
Yue Liu		 1013bp
Bba_K1020002 Coding alkane regulatory protein ALKR Boxuan Zeng 927bp
Bba_K1020003 Reporter AlkR -PSB1C3-Low constitutive Yue Liu 1125bp
Bba_K1020004 Composite Biosensor for alkane detection Yue Liu
Su Wang		 2009bp
Bba_K1020006 Coding YbhGFSR Boxuan Zeng
Yang Wu 		4971bp
Bba_K1020007 Composite AlkR -PSB1C3-High constitutive Yue Liu 1125bp
Bba_K1020008 Composite alkane producing module Boxuan Zeng
Yue Liu		 1809bp
Bba_K1020009 Composite YbhGFSR operon Boxuan Zeng 5034bp
Bba_K1020010 Coding TetA gene under the control of PalkM Boxuan Zeng
Shoujie Sun		 1529bp
Bba_K1020011 Composite High expressed Alkane Selector for intracellular alkane production Boxuan Zeng
Qingyi Deng 		2662bp

Others

Results for Agarose Gel Electrophoresis:

gel 1

Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT


gel 2

Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT

gel 3

Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT

gel 4

Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3


gel 5

Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3

gel 6

Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3

Characterization for our construction:


Figure 7. The visible color change of the strain cultured in 3ML M9 medium with different induced for 12 hours.
Figure 8. Strain one contains alkane producer part(left),another contains none-producer part (right) .

Retrieved from "http://2013.igem.org/Team:Tianjin/Data"