Team:Tianjin/Data

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                 <a href="https://2013.igem.org/Team:Tianjin/Human_Practice/NewTeamsGuidance">New Teams Guidance</a>
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                 <a href="https://2013.igem.org/Team:Tianjin/Human_Practice/iGEMLeading-in">iGEM Leading-in</a>
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                        <a href="">iGEM Handbook</a>
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                <a href="https://2013.igem.org/Team:Tianjin/Human_Practice/Collaboration ">Collaboration</a>
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                        <a href="">The 1st Model iGEM</a>
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                        <a href="">Tsinghua University</a>
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                        <a href="">Ocean University of China</a>
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                <a href="https://2013.igem.org/Team:Tianjin/Human_Practice/SocietyOutreach ">Society Outreach</a>
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                        <a href="">PetroChina Dagang Petrochemical Company</a>
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                        <a href=""><li>Communicate with Research Institute</li></a>
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                 <a href="https://2013.igem.org/Main_Page" title="Main Page"><img src="https://static.igem.org/mediawiki/2013/7/75/IGEM-logo-blue.png" width="200px" height="200px" border="none" style="margin-top:80px;"/></a>  
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<td>Composite</td>
<td>Composite</td>
<td> Alkane Marker--Device for detection of various alkanes</td>
<td> Alkane Marker--Device for detection of various alkanes</td>
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<td>Boxuan Zeng,Yue Liu </td>
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<td>Boxuan Zeng<br/>Yue Liu </td>
<td> 2146bp</td>
<td> 2146bp</td>
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<td> Composite</td>
<td> Composite</td>
<td> Alkane Selector for intracellular alkane production</td>
<td> Alkane Selector for intracellular alkane production</td>
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<td> Boxuan Zeng,Yue Liu </td>
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<td> Boxuan Zeng<br/>Yue Liu </td>
<td>2662bp </td>
<td>2662bp </td>
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==Tianjin 2012 iGEM Team Parts Sandbox==
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==Tianjin 2013 iGEM Team Parts Sandbox==
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<td>Reporter</td>
<td>Reporter</td>
<td>PalkM promoter with reporter gene</td>
<td>PalkM promoter with reporter gene</td>
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<td>Boxuan Zeng,Yue Liu</td>
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<td>Boxuan Zeng<br/>Yue Liu</td>
<td>1013bp</td>
<td>1013bp</td>
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<td> Composite</td>
<td> Composite</td>
<td> Biosensor for alkane detection</td>
<td> Biosensor for alkane detection</td>
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<td> Yue Liu,Su Wang</td>
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<td> Yue Liu<br/>Su Wang</td>
<td> 2009bp</td>
<td> 2009bp</td>
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<td>Coding </td>
<td>Coding </td>
<td>YbhGFSR </td>
<td>YbhGFSR </td>
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<td>Boxuan Zeng,Yang Wu  </td>
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<td>Boxuan Zeng<br/>Yang Wu  </td>
<td> 4971bp</td>
<td> 4971bp</td>
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<td> Composite</td>
<td> Composite</td>
<td>alkane producing module </td>
<td>alkane producing module </td>
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<td> Boxuan Zeng,Yue Liu</td>
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<td> Boxuan Zeng<br/>Yue Liu</td>
<td>1809bp </td>
<td>1809bp </td>
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<td>Coding </td>
<td>Coding </td>
<td>TetA gene under the control of PalkM </td>
<td>TetA gene under the control of PalkM </td>
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<td> Boxuan Zeng,Shoujie Sun</td>
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<td> Boxuan Zeng<br/>Shoujie Sun</td>
<td> 1529bp</td>
<td> 1529bp</td>
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<td> Composite</td>
<td> Composite</td>
<td>High expressed Alkane Selector for intracellular alkane production </td>
<td>High expressed Alkane Selector for intracellular alkane production </td>
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<td>Boxuan Zeng,Qingyi Deng </td>
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<td>Boxuan Zeng<br/>Qingyi Deng </td>
<td>2662bp </td>
<td>2662bp </td>
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=Others=
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----
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'''Results for Agarose Gel Electrophoresis:'''
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[[File:Tju-gel-1.1.1.jpg|center|330px|gel 1]]
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<b>Figure 1.</b>&nbsp;AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT</div>
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[[File:Tju-gel-1.1.2.jpg|center|320px|gel 2]]
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<b>Figure 2.</b>&nbsp;AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT</div>
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[[File:Tju-gel-1.1.3.jpg|center|330px|gel 3]]
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<b>Figure 3.</b>&nbsp;J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT</div>
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[[File:Tju-gel-1.2.1.jpg|center|500px|gel 4]]
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<b>Figure 4.</b>&nbsp;J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3</div>
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[[File:Tju-gel-1.2.2.jpg|center|460px|gel 5]]
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<b>Figure 5.</b>&nbsp;J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3</div>
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[[File:Tju-gel-1.3.jpg|center|330px|gel 6]]
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<b>Figure 6.</b>&nbsp;J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3</div>
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'''Characterization for our construction:'''
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<img src="https://static.igem.org/mediawiki/2013/3/3e/TJU-PROJECT-C3.jpg"/>
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<b>Figure 7.</b>&nbsp;The visible color change of the strain cultured in 3ML M9 medium with different induced for 12 hours.</div>
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<b>Figure 8.</b>&nbsp;Strain one contains alkane producer part(left),another contains none-producer part (right) .</div>

Latest revision as of 01:45, 29 October 2013

Contents

BioBrick

Favorite Tianjin 2013 iGEM Team Parts

Name Type Description Designer Length
Bba_K1020005 Composite Alkane Marker--Device for detection of various alkanes Boxuan Zeng
Yue Liu 		2146bp
Bba_K1020012 Composite Alkane Selector for intracellular alkane production Boxuan Zeng
Yue Liu 		2662bp


Tianjin 2013 iGEM Team Parts Sandbox

Name Type Description Designer Length
Bba_K1020001 Reporter PalkM promoter with reporter gene Boxuan Zeng
Yue Liu		 1013bp
Bba_K1020002 Coding alkane regulatory protein ALKR Boxuan Zeng 927bp
Bba_K1020003 Reporter AlkR -PSB1C3-Low constitutive Yue Liu 1125bp
Bba_K1020004 Composite Biosensor for alkane detection Yue Liu
Su Wang		 2009bp
Bba_K1020006 Coding YbhGFSR Boxuan Zeng
Yang Wu 		4971bp
Bba_K1020007 Composite AlkR -PSB1C3-High constitutive Yue Liu 1125bp
Bba_K1020008 Composite alkane producing module Boxuan Zeng
Yue Liu		 1809bp
Bba_K1020009 Composite YbhGFSR operon Boxuan Zeng 5034bp
Bba_K1020010 Coding TetA gene under the control of PalkM Boxuan Zeng
Shoujie Sun		 1529bp
Bba_K1020011 Composite High expressed Alkane Selector for intracellular alkane production Boxuan Zeng
Qingyi Deng 		2662bp

Others

Results for Agarose Gel Electrophoresis:

gel 1

Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT


gel 2

Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT

gel 3

Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT

gel 4

Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3


gel 5

Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3

gel 6

Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3

Characterization for our construction:


Figure 7. The visible color change of the strain cultured in 3ML M9 medium with different induced for 12 hours.
Figure 8. Strain one contains alkane producer part(left),another contains none-producer part (right) .

Retrieved from "http://2013.igem.org/Team:Tianjin/Data"