Team:Tianjin/Data

From 2013.igem.org

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==Tianjin 2012 iGEM Team Parts Sandbox==
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'''Results for Agarose Gel Electrophoresis:'''
[[File:Tju-gel-1.1.1.jpg|center|330px|gel 1]]
[[File:Tju-gel-1.1.1.jpg|center|330px|gel 1]]
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<b>Figure 1.</b>&nbsp;AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT</div>
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[[File:Tju-gel-1.1.2.jpg|center|320px|gel 2]]
[[File:Tju-gel-1.1.2.jpg|center|320px|gel 2]]
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<b>Figure 2.</b>&nbsp;AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT</div>
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[[File:Tju-gel-1.1.3.jpg|center|330px|gel 3]]
[[File:Tju-gel-1.1.3.jpg|center|330px|gel 3]]
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<b>Figure 3.</b>&nbsp;J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT</div>
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[[File:Tju-gel-1.2.1.jpg|center|500px|gel 4]]
[[File:Tju-gel-1.2.1.jpg|center|500px|gel 4]]
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<b>Figure 4.</b>&nbsp;J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3</div>
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[[File:Tju-gel-1.2.2.jpg|center|460px|gel 5]]
[[File:Tju-gel-1.2.2.jpg|center|460px|gel 5]]
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<b>Figure 5.</b>&nbsp;J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3</div>
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[[File:Tju-gel-1.3.jpg|center|330px|gel 6]]
[[File:Tju-gel-1.3.jpg|center|330px|gel 6]]
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[[File:1000.jpg|center|700px]]
 
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<b>Figure 6.</b>&nbsp;J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3</div>
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'''Characterization for our construction:'''
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<img src="https://static.igem.org/mediawiki/2013/3/3e/TJU-PROJECT-C3.jpg"/>
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<b>Figure 7.</b>&nbsp;The visible color change of the strain cultured in 3ML M9 medium with different induced for 12 hours.</div>
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<DIV ALIGN="CENTER"><img src="https://static.igem.org/mediawiki/2013/f/fe/QQ图片20131029093339.jpg" width="400px"/></DIV>
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<b>Figure 8.</b>&nbsp;Strain one contains alkane producer part(left),another contains none-producer part (right) .</div>
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Latest revision as of 01:45, 29 October 2013

Contents

BioBrick

Favorite Tianjin 2013 iGEM Team Parts

Name Type Description Designer Length
Bba_K1020005 Composite Alkane Marker--Device for detection of various alkanes Boxuan Zeng
Yue Liu 		2146bp
Bba_K1020012 Composite Alkane Selector for intracellular alkane production Boxuan Zeng
Yue Liu 		2662bp


Tianjin 2013 iGEM Team Parts Sandbox

Name Type Description Designer Length
Bba_K1020001 Reporter PalkM promoter with reporter gene Boxuan Zeng
Yue Liu		 1013bp
Bba_K1020002 Coding alkane regulatory protein ALKR Boxuan Zeng 927bp
Bba_K1020003 Reporter AlkR -PSB1C3-Low constitutive Yue Liu 1125bp
Bba_K1020004 Composite Biosensor for alkane detection Yue Liu
Su Wang		 2009bp
Bba_K1020006 Coding YbhGFSR Boxuan Zeng
Yang Wu 		4971bp
Bba_K1020007 Composite AlkR -PSB1C3-High constitutive Yue Liu 1125bp
Bba_K1020008 Composite alkane producing module Boxuan Zeng
Yue Liu		 1809bp
Bba_K1020009 Composite YbhGFSR operon Boxuan Zeng 5034bp
Bba_K1020010 Coding TetA gene under the control of PalkM Boxuan Zeng
Shoujie Sun		 1529bp
Bba_K1020011 Composite High expressed Alkane Selector for intracellular alkane production Boxuan Zeng
Qingyi Deng 		2662bp

Others

Results for Agarose Gel Electrophoresis:

gel 1

Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT


gel 2

Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT

gel 3

Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT

gel 4

Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3


gel 5

Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3

gel 6

Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3

Characterization for our construction:


Figure 7. The visible color change of the strain cultured in 3ML M9 medium with different induced for 12 hours.
Figure 8. Strain one contains alkane producer part(left),another contains none-producer part (right) .

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