Team:Hong Kong CUHK/vs

From 2013.igem.org

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  <p2>  
  <p2>  
<p align="center"><img src="https://static.igem.org/mediawiki/2013/2/29/VS.JPG"/></p>
<p align="center"><img src="https://static.igem.org/mediawiki/2013/2/29/VS.JPG"/></p>
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<p><h4>Voltage Switch (Protein Domain <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092006">BBa_K1092006</a>)</h4></p>
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<p><h4>Voltage Sensor Peptide (Protein Domain <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092006">BBa_K1092006</a>)</h4></p>
  <p align="center"><img src="https://static.igem.org/mediawiki/2013/6/6e/S1.png"></p>
  <p align="center"><img src="https://static.igem.org/mediawiki/2013/6/6e/S1.png"></p>
   <p align="left">
   <p align="left">
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   It is a protein  domain found in a transmembrane potassium ion channel from <em>Aeropyrum pernix</em>, which is highly voltage dependent. It contain positive charges  from the 4 arginine residues, and also numerous leucine residues which give it  high hydrophobicity. All these together make it an excellent  voltage sensing peptide in the transmembrane region. Study has shown that this helical voltage sensor peptide is capable of moving across the membrane under external voltage, which is the key to the voltage dependence of the ion channel  (PMID: 12721618). We make use of this feature to construct a novel voltage switch (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092007">BBa_K1092007</a> &amp; <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092008">BBa_K1092008</a>), which consist of the PDZ domain (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092102">BBa_K1092102</a>), PDZ ligand (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092101">BBa_K1092101</a>), this voltage sensor peptide itself. To further adapt this voltage sensor peptide into our project, we added in two (GGGGS)3 flexible linker which serve to link other protein domains, and a (PE)5 linker which serve as a rigid rod that project from the sensor peptide to elongate it  and increase the separation distance.</p>
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   Voltage sensor peptide, from <em>Aeropyrum pernix</em>, is a protein domain found in a transmembrane potassium ion channel. It contains positively-charged 4 arginine residues, and also numerous hydrophobic leucine residues. Study has shown that this helical voltage sensor peptide is capable of moving within the membrane under external voltage. To further adapt this voltage sensor peptide into our project, we added in glycine serine flexible linker, and polyproline rigid linker.</p>
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  <p>Part-only sequencing result: <br />
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    (                      (GGGGS)3  linker                         )(<br />
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    GGTGGTGGTG  GTTCTGGTGG TGGTGGTTCT GGTGGTGGTG GTTCTCTGGG <br />
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    Voltage sensor  peptide<br />
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    TCTGTTTCGT CTGGTGCGCC  TGCTGCGTTT TCTGCGTATT CTGCTGATTA <br />
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    )(                                      (Pe)5 linker           )(<br />
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    TTTGCCCGGA ACCGGAACCG  GAACCAGAAC CAGAAGGTGG TGGTGGTTCT <br />
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    (GGGGS)3 linker                           )<br />
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    GGTGGTGGTG GTTCTGGTGG  TGGTGGTTCT</p>
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   <p align="left">Note: part name should be voltage sensor peptide, not  voltage switch </p>
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   <p>&nbsp;</p>
   <p>&nbsp;</p>
   <h4>ssDsbA-PDZ Ligand-Voltage Switch (Protein Domain <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092007">BBa_K1092007</a>)</h4>
   <h4>ssDsbA-PDZ Ligand-Voltage Switch (Protein Domain <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092007">BBa_K1092007</a>)</h4>
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   <h3>T7  – RBS – Voltage Sensor Peptide (Device <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092013">BBa_K1092013</a>)</h3>
   <h3>T7  – RBS – Voltage Sensor Peptide (Device <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092013">BBa_K1092013</a>)</h3>
   <p align="center"><img src="https://static.igem.org/mediawiki/2013/7/78/20131.png"><img src="https://static.igem.org/mediawiki/2013/7/74/20132.png"></p>
   <p align="center"><img src="https://static.igem.org/mediawiki/2013/7/78/20131.png"><img src="https://static.igem.org/mediawiki/2013/7/74/20132.png"></p>
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<p>It is a protein domain found in a transmembrane potassium ion channel from Aeropyrum pernix, which is highly voltage dependent. It contain positive charges from the 4 arginine residues, and also numerous leucine residues which give it high hydrophobicity. All these together make it an excellent voltage sensing peptide in the transmembrane region. Study has shown that this helical voltage sensor peptide is capable of moving across the membrane under external voltage, which is the key to the voltage dependence of the ion channel (PMID: 12721618). We make use of this feature to construct a novel voltage switch (BBa_K1092007 &amp; BBa_K1092008), which consist of the PDZ domain (BBa_K1092102), PDZ ligand (BBa_K1092101), this voltage sensor peptide itself. To further adapt this voltage sensor peptide into our project, we added in two (GGGGS)3 flexible linker which serve to link other protein domains, and a (PE)5 linker which serve as a rigid rod that project from the sensor peptide to elongate it and increase the separation distance.</p>
   <p>&nbsp;</p>
   <p>&nbsp;</p>
   <h4>T7 - RBS - PDZ Domain - Voltage Sensor – RFP C Terminus (Protein Domain <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092024">BBa_K1092024</a>)</h4>
   <h4>T7 - RBS - PDZ Domain - Voltage Sensor – RFP C Terminus (Protein Domain <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1092024">BBa_K1092024</a>)</h4>

Latest revision as of 02:28, 29 October 2013

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