Team:Tianjin/Project/Futurework

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                         <a href="https://2013.igem.org/Team:Tianjin/Project/Introduction #Inspiration">Background</a>  
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                         <a href="https://2013.igem.org/Team:Tianjin/Project/Introduction #Background">Background</a>  
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                         <a href="https://2013.igem.org/Team:Tianjin/Project/Introduction #Supplement_information">Supplementary Information</a>  
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                         <a href="https://2013.igem.org/Team:Tianjin/Project/Introduction #Supplement_information">Supplement Information</a>  
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                 <a href="https://2013.igem.org/Team:Tianjin/Project/Application">Alk-Selector And Direct Evolution</a>  
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                 <a href="https://2013.igem.org/Team:Tianjin/Project/Alk-Selector&DirectEvolution">Alk-Selector & Direct Evolution</a>  
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                         <a href="#">Biosensor optimization</a>  
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                         <a href="https://2013.igem.org/Team:Tianjin/Project/Futurework #Summary">Summary</a>  
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                         <a href="#">Direct evolution for pathway optimization</a>  
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                         <a href="https://2013.igem.org/Team:Tianjin/Project/Futurework #Future_work">Future work</a>  
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= Summary:=
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= Summary=
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In this summer, we construct a new alkane sensing system AlkR-PalkM, all the achievements are based on this system.
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In this summer, we constructed a new alkane sensing system AlkR-PalkM, all the achievements are based on this system.
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#1.Based on the system of AlkR-PalkM , we use this to build an Alk-Sensor based on the system of AlkR-PalkM which can detect the alkane which is produced by engineered E. coli.
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1.We built an Alk-Sensor based on the system of AlkR-PalkM which can detect the alkanes which are produced by engineered <i>E. coli</i>.
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#2.We characterized the Alk-Sensor of different aspects.
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2.We characterized the Alk-Sensor of different aspects.
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#3.Based on Alk-Sensor, we constructed Alk-Selector for direct evolution
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3.Based on Alk-Sensor, we constructed Alk-Selector for directed evolution.
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#4.We used Alk-Selector to optimize the function of NPDC.
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4.We used Alk-Selector to optimize the function of NPDC.
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= Future work:=
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= Future work=
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All parts are also not perfect, needed to be optimized.
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All parts are still not perfect, needed to be optimized.
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#1.It may be necessary to mutate PalkM to reduce the leakage of the system.
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1.It may be necessary to mutate PalkM to reduce the leakage of the system.
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#2.Different reporter needed to be tried to get different relationship between input and output.
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2.Different reporters needed to be tried to get different relationships between input and output.
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#3.Mutate AlkR may promote the interaction between AlkR and Alkane, and improve the function of AlkR-PalkM system.
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3.Mutating AlkR will probably promote the interaction between AlkR and alkanes, and improve the function of AlkR-PalkM system.
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#4.Addition of gene related to biosufactant biosynthesis may increasing the sensing range of the alkane
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4.Addition of genes related to biosufactant biosynthesis may increase the sensing range of Alk-Sensor to alkanes.
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#5.For biofuel synthetic, Alk-Selector provide the more opportunities to engineered the strain. Many method related to build a library can be used in this field with the help of Alk-Selector.
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5.For biofuel synthesis, Alk-Selector provides more opportunities to engineer the strains. Many methods related to building a library can be used in this field with the help of Alk-Selector.
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<a href="https://2013.igem.org/Team:Tianjin/Project/Introduction"><img src="#" width="120px" height="120px" align="left" alt="Introduction" title="Introduction"/></a>
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<a href="https://2013.igem.org/Team:Tianjin/Project/Introduction"><img src="https://static.igem.org/mediawiki/2013/d/d3/TJU-project-banner01.jpg" width="120px" height="120px" align="left" alt="Introduction" title="Introduction"/></a>
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<a href="https://2013.igem.org/Team:Tianjin/Project/Design&Construction"><img src="#" width="120px" height="120px" align="left" alt="Design & Construction" title="Design & Construction"/></a>
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<a href="https://2013.igem.org/Team:Tianjin/Project/Design&Construction"><img src="https://static.igem.org/mediawiki/2013/a/a2/TJU-project-banner02.jpg" width="120px" height="120px" align="left" alt="Design & Construction" title="Design & Construction"/></a>
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<a href="https://2013.igem.org/Team:Tianjin/Project/Characterization"><img src="#" width="120px" height="120px" align="left" alt="Characterization" title="Characterization"/></a>
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<a href="https://2013.igem.org/Team:Tianjin/Project/Characterization"><img src="https://static.igem.org/mediawiki/2013/9/9c/TJU-project-banner03.jpg" width="120px" height="120px" align="left" alt="Characterization" title="Characterization"/></a>
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<a href="https://2013.igem.org/Team:Tianjin/Project/Application"><img src="#" width="120px" height="120px" align="left" alt="Application" title="Application"/></a>
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<a href="https://2013.igem.org/Team:Tianjin/Project/Alk-Selector&DirectEvolution"><img src="https://static.igem.org/mediawiki/2013/9/98/TJU-project-banner04.jpg" width="120px" height="120px" align="left" alt="Alk-Selector And Direct Evolution" title="Alk-Selector And Direct Evolution"/></a>
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<a href="https://2013.igem.org/Team:Tianjin/Project/Futurework"><img src="#" width="120px" height="120px" align="left" alt="Futurework" title="Futurework"/></a>
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<a href="https://2013.igem.org/Team:Tianjin/Project/Futurework"><img src="https://static.igem.org/mediawiki/2013/a/a2/TJU-project-banner05.jpg" width="120px" height="120px" align="left" alt="Futurework" title="Futurework"/></a>
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Latest revision as of 02:57, 29 October 2013


Future work


Summary


In this summer, we constructed a new alkane sensing system AlkR-PalkM, all the achievements are based on this system.

1.We built an Alk-Sensor based on the system of AlkR-PalkM which can detect the alkanes which are produced by engineered E. coli.

2.We characterized the Alk-Sensor of different aspects.

3.Based on Alk-Sensor, we constructed Alk-Selector for directed evolution.

4.We used Alk-Selector to optimize the function of NPDC.


Future work


All parts are still not perfect, needed to be optimized.

1.It may be necessary to mutate PalkM to reduce the leakage of the system.

2.Different reporters needed to be tried to get different relationships between input and output.

3.Mutating AlkR will probably promote the interaction between AlkR and alkanes, and improve the function of AlkR-PalkM system.

4.Addition of genes related to biosufactant biosynthesis may increase the sensing range of Alk-Sensor to alkanes.

5.For biofuel synthesis, Alk-Selector provides more opportunities to engineer the strains. Many methods related to building a library can be used in this field with the help of Alk-Selector.






Introduction Design & Construction Characterization Alk-Selector And Direct Evolution Futurework

Retrieved from "http://2013.igem.org/Team:Tianjin/Project/Futurework"