Team:Cornell/notebook/plasmids

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Latest revision as of 03:17, 29 October 2013

Cornell University Genetically Engineered Machines

Plasmids

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Plasmid Name	Part	Contents/Description	Origin	Backbone	Antibiotic Resistance	Antifungal Resistance
pC13A	BBa_K523022	crtEBI	Kit Plate 1 8P	pSB1C3	Chloramphenicol	N/A
pC13B	BBa_K539119	crtY	Kit Plate 1 12K	pSB1C3	Chloramphenicol	N/A
pA13C	pIVEX2.3d	T7 promoter/terminator	Luo Lab	pIVEX2.3d	Ampicillin	N/A
pAK13D	BBa_I712074	T7 promoter	Kit Plate 5 6N	pSB1AK8	Ampicillin	N/A
pC13E	BBa_K145001	T7 polymerase	Kit Plate 4 2C	pSB1C3	Chloramphenicol	N/A
pC13F	BBa_J04450	mRFP in pSB1C3	T.E. Kit	pSB1C3	Chloramphenicol	N/A
pAh13G	pUCATPH	PtrpC, hph, TtrpC	Turgeon lab	pUCATPH	Ampicillin	Hygromycin
pAg13H	pNG	Pgpd (C. h.), nptII	Turgeon lab	pNG	Ampicillin	Geneticin
pC13I	BBa_K1021000	crtE	A	pSB1C3	Chloramphenicol	N/A
pC13J	invalid	rbs + crtE	A	pSB1C3	Chloramphenicol	N/A
pC13K	BBa_K118003	crtI	Kit Plate 3 2B	pSB1C3	Chloramphenicol	N/A
pC13L	BBa_K118005	rbs + crtI	Kit Plate 4 4A	pSB1C3	Chloramphenicol	N/A
pC13M	BBa_K118002	crtB	Kit Plate 3 17N	pSB1C3	Chloramphenicol	N/A
pC13N	BBa_K118006	rbs + crtB	Kit Plate 3 17P	pSB1C3	Chloramphenicol	N/A
pC13O	BBa_I742155	rbs + crtY	Kit Plate 5 17P	pSB1C3	Chloramphenicol	N/A
pC13P	BBa_K1021007	PtrpC	G	pSB1C3	Chloramphenicol	N/A
pC13Q	BBa_K1021008	TtrpC	G	pSB1C3	Chloramphenicol	N/A
pCg13R	invalid	nptII	H	pSB1C3	Chloramphenicol	Geneticin
pCg13S	BBa_K1021001	nptIIBB	R	pSB1C3	Chloramphenicol	Geneticin
pAb13T	pBARGPE1	PtrpC, bar, TtrpC, PgpdA, lox	Fungal Genetics Stock Center	pBARGPE1	Ampicillin	Phosphonothricin
pCg13U	invalid	nptIIBB + TtrpC	S, Q	pSB1C3	Chloramphenicol	Geneticin
pCh13V	invalid	hph	G, F	pSB1C3	Chloramphenicol	Hygromycin
pCh13W	BBa_K1021002	hphBB	V	pSB1C3	Chloramphenicol	Hygromycin
pCp13X	BBa_K1021003	bar	T, F	pSB1C3	Chloramphenicol	Phosphonothricin
pCg13Y	BBa_K1021015	PtrpC + nptIIBB	P, S	pSB1C3	Chloramphenicol	Geneticin
pC13Z	BBa_K082003	GFP(+LVA)	Kit Plate 3 20N	pSB1C3	Chloramphenicol	N/A
pAK13AA	invalid	PT7 + crtE	D, A	pSB1AK8	Ampicillin & Kanamycin	N/A
pAK13AB	invalid	PT7 + crtB	D, M	pSB1AK8	Ampicillin & Kanamycin	N/A
pAK13AC	invalid	PT7 + crtI	D, K	pSB1AK8	Ampicillin & Kanamycin	N/A
pAK13AD	invalid	PT7 + crtY	D, B	pSB1AK8	Ampicillin & Kanamycin	N/A
pC13AE	BBa_K1021016	PT7 + crtE	F, AA	pSB1C3	Chloramphenicol	N/A
pC13AF	BBa_K1021017	PT7 + crtB	F, AB	pSB1C3	Chloramphenicol	N/A
pC13AG	BBa_K1021018	PT7 + crtI	F, AC	pSB1C3	Chloramphenicol	N/A
pC13AH	BBa_K1021019	PT7 + crtY	F, AD	pSB1C3	Chloramphenicol	N/A
pC13AI	BBa_J61046	lox site	Kit Plate 3 3L	pSB1C3	Chloramphenicol	N/A
pA13AJ	BBa_J61046	lox site	Kit Plate 5 24B	pSB1A2	Ampicillin	N/A
pC13AK	BBa_E1010	mRFP cds only	Kit Plate 3 12N	pSB1C3	Chloramphenicol	N/A
pK13AL	BBa_E1010	mRFP cds only	Kit Plate 5 18F	pSB2K3	Kanamycin	N/A
pA13AM	BBa_J23100	strongest Anderson constitutive promoter with mRFP downstream	Kit Plate 5 18C	BBa_J61002	Ampicillin & Kanamycin	N/A
pAK13AN	invalid	PT7+GFP	D, Z	pSB1AK8	Ampicillin & Kanamycin	N/A
pAK13AO	invalid	PT7+mRFP	D, AK/AL	pSB1AK8	Ampicillin & Kanamycin	N/A
pAK13AP	invalid	PT7+bar	D, X	pSB1AK8	Ampicillin & Kanamycin	Phosphonothricin
pC13AQ	BBa_K1021020	PT7+GFP	AN, F	pSB1C3	Chloramphenicol	N/A
pC13AR	BBa_K1021021	PT7+mRFP	AO, F	pSB1C3	Chloramphenicol	N/A
pC13AS	BBa_K1021022	PT7+bar	AP, F	pSB1C3	Chloramphenicol	Phosphonothricin
pC13AT	BBa_K1021023	PtrpC+GFP	P, Z	pSB1C3	Chloramphenicol	N/A
pC13AU	BBa_K1021024	PtrpC+mRFP	P, AK/AL	pSB1C3	Chloramphenicol	N/A
pC13AV	BBa_K1021025	PtrpC+bar	P, X	pSB1C3	Chloramphenicol	Phosphonothricin
pA13AW	invalid	Anderson promoter + T7Pol	AM, E	BBa_J61002	Ampicillin	N/A
pK13AX	invalid	Anderson promoter + T7Pol	AW, AL (backbone)	pSB2K3	Kanamycin	N/A
pC13AY	unsubmitted	PtrpC+T7Pol	P, E	pSB1C3	Chloramphenicol	N/A
pC13AZ	BBa_K1021026	Anderson promoter + T7Pol	AW, F	pSB1C3	Chloramphenicol	N/A
pC13BA	BBa_K1021004	afp1	G-block	pSB1C3	Chloramphenicol	N/A
pAK13BB	invalid	PT7+nptIIBB	D, S	pSB1AK8	Ampicillin & Kanamycin	Geneticin
pC13BC	BBa_K1021032	PT7+nptIIBB	BB, F	pSB1C3	Chloramphenicol	Geneticin
pC13BD	unsubmitted	PtrpC+crtI	P, K	pSB1C3	Chloramphenicol	N/A
pA13BE	pHNU3pelA	PpelA, flanked by KpnI/BamHI	Turgeon lab	pHPG	Ampicillin	N/A
pC13BF	BBa_K1021009	PpelA	BE, F	pSB1C3	Chloramphenicol	N/A
pC13BG	BBa_K1021010	A. nidulans PgpdA	T, F	pSB1C3	Chloramphenicol	N/A
pC13BH	invalid	G. lucidum PgpdA	Ganoderma genome, F	pSB1C3	Chloramphenicol	N/A
pC13BI	unsubmitted	G. lucidum PgpdA (BB)	BH	pSB1C3	Chloramphenicol	N/A
pC13BJ	BBa_K1021011	100bp left region of homology (Ganoderma gpd gene)	Ganoderma genome, F	pSB1C3	Chloramphenicol	N/A
pC13BK	BBa_K1021012	200bp left region of homology (Ganoderma gpd gene)	Ganoderma genome, F	pSB1C3	Chloramphenicol	N/A
pC13BL	BBa_K1021013	100bp right region of homology (Ganoderma gpd gene)	Ganoderma genome, F	pSB1C3	Chloramphenicol	N/A
pC13BM	BBa_K1021014	200bp right region of homology (Ganoderma gpd gene)	Ganoderma genome, F	pSB1C3	Chloramphenicol	N/A
pC13BN	unsubmitted	lox [cutsite 1] [cutsite 2] lox	AI	pSB1C3	Chloramphenicol	N/A
pC13BO	abandoned	short homology regions flanking two unique cutsites	BJ, BL	pSB1C3	Chloramphenicol	N/A
pC13BP	abandoned	long homology regions flanking two unique cutsites	BK, BM	pSB1C3	Chloramphenicol	N/A
pC13BQ	BBa_K1021027	A. nidulans PgpdA + nptIIBB	BG, S	pSB1C3	Chloramphenicol	Geneticin
pC13BR	BBa_K1021028	A. nidulans PgpdA + hphBB	BG, W	pSB1C3	Chloramphenicol	Hygromycin
pC13BS	unsubmitted	G. lucidum PgpdABB + nptIIBB	BI, S	pSB1C3	Chloramphenicol	Geneticin
pC13BT	unsubmitted	G. lucidum PgpdABB + hphBB	BI, W	pSB1C3	Chloramphenicol	Hygromycin
pAK13BU	invalid	PT7 + hphBB	D, W	pSB1AK8	Ampicillin & Kanamycin	Hygromycin
pC13BV	BBa_K1021033	PT7 + hphBB	BU, F	pSB1C3	Chloramphenicol	Hygromycin
pA13BW	pBS185CMV-Cre	pBS185 CMV Cre	Addgene	pBS185	Ampicillin	N/A
pC13BX	BBa_K1021005	Cre recombinase	BW	pSB1C3	Chloramphenicol	N/A
pC13BY	unsubmitted	PpelA + Cre	BF, BX	pSB1C3	Chloramphenicol	N/A
pC13BZ	unsubmitted	PpelA + GFP	BF, Z	pSB1C3	Chloramphenicol	N/A
pC13CA	unsubmitted	PpelA + mRFP	BF, AL	pSB1C3	Chloramphenicol	N/A
pC13CB	unsubmitted	PT7 + GFP + PtrpC + nptIIBB	Y, AQ	pSB1C3	Chloramphenicol	Geneticin
pC13CC	BBa_K1021029	PtrpC + nptIIBB + TtrpC	Y, Q	pSB1C3	Chloramphenicol	Geneticin
pC13CD	BBa_K1021030	PtrpC + hphBB	P, W	pSB1C3	Chloramphenicol	Hygromycin
pC13CE	BBa_K1021034	PtrpC + hphBB +TtrpC	CD, Q	pSB1C3	Chloramphenicol	Hygromycin
pC13CF	unsubmitted	A. n. PgpdA + GFP	BG, Z	pSB1C3	Chloramphenicol	N/A
pC13CG	BBa_K1021031	A. n. PgpdA + mRFP	BG, AK/AL	pSB1C3	Chloramphenicol	N/A
pC13CH	unsubmitted	G. l. PgpdA + GFP	BH, Z	pSB1C3	Chloramphenicol	N/A
pC13CI	unsubmitted	G. I. PgpdA + mRFP	BH, AK/AL	pSB1C3	Chloramphenicol	N/A
pC13CJ	BBa_I742111	Limonene synthase	Kit plate 4	pSB1C3	Chloramphenicol	N/A
pAK13CK	unsubmitted	PT7+Limonene Synthase	CJ, D	pSB1AK8	Ampicillin & Kanamycin	N/A
pC13CL	BBa_K1021006	Cht1_2 in pSB1C3	IDT Geneblock	pSB1C3	Chloramphenicol	N/A
pC13CM	unsubmitted	PpelA + Cht1_2	CL, BF	pSB1C3	Chloramphenicol	N/A
pS13CN	BBa_K112306	Lambda Holin	Kit plate 3 2012 BBa_K112306	BBa_K112950	Spectinomycin	N/A
pC13CO	unsubmitted	Lambda Holin in pSB1C3	CN	pSB1C3	Chloramphenicol	N/A
pC13CP	unsubmitted	PpelA+Lambda Holin	CO	pSB1C3	Chloramphenicol	N/A
pAK13CQ	invalid	PT7 + rbs + crtE	D, J	pSB1AK8	Ampicillin & Kanamycin	N/A
pAK13CR	invalid	PT7 + rbs + crtB	D, N	pSB1AK8	Ampicillin & Kanamycin	N/A
pAK13CS	invalid	PT7 + rbs + crtI	D, L	pSB1AK8	Ampicillin & Kanamycin	N/A
pAK13CT	invalid	PT7 + rbs + crtY	D, O	pSB1AK8	Ampicillin & Kanamycin	N/A
pC13CU	unsubmitted	PT7 + rbs + crtE	F, CQ	pSB1C3	Chloramphenicol	N/A
pC13CV	unsubmitted	PT7 + rbs + crtB	F, CR	pSB1C3	Chloramphenicol	N/A
pC13CW	unsubmitted	PT7 + rbs + crtI	F, CS	pSB1C3	Chloramphenicol	N/A
pC13CX	unsubmitted	PT7 + rbs + crtY	F, CT	pSB1C3	Chloramphenicol	N/A
pAK13CY	invalid	PT7 + rbs + GFP	D, DB	pSB1AK8	Ampicillin & Kanamycin	N/A
pA13CZ	BBa_B0034	RBS (strong)	Kit Plate 5, Well 2M	pSB1A2	Ampicillin	N/A
pAK13DA	invalid	PT7 + rbs + mRFP	D, DC	pSB1AK8	Ampicillin & Kanamycin	N/A
pA13DB	invalid	RBS+GFP	CZ, Z	pSB1A2	Ampicillin	N/A
pA13DC	invalid	RBS+mRFP	CZ, AK	pSB1A2	Ampicillin	N/A
pC13DD	unsubmitted	PT7+Limonene Synthase	F, CK	pSB1C3	Chloramphenicol	N/A
pC13DE	unsubmitted	lox-PtrpC-nptIIBB-lox	Y, BN	pSB1C3	Chloramphenicol	Geneticin
pC13DF	unsubmitted	lox-A.PgpdA-nptIIBB-lox	BN, BQ	pSB1C3	Chloramphenicol	Geneticin
pC13DG	unsubmitted	lox-A.PgpdA-hphBB-lox	BN, BR	pSB1C3	Chloramphenicol	Hygromycin
pC13DH	unsubmitted	lox-PtrpC-hphBB-lox	BN, CD	pSB1C3	Chloramphenicol	Hygromycin
pC13DI	unsubmitted	PtrpC + hphBB + GFP	CD, Z	pSB1C3	Chloramphenicol	Hygromycin
pC13DJ	unsubmitted	PT7 + crtB + PT7 + crtI	AF, AG	pSB1C3	Chloramphenicol	N/A
pC13DK	unsubmitted	PT7 + crtE + PT7 + crtB + PT7 + crtI	DJ, AE	pSB1C3	Chloramphenicol	N/A
pC13DL	unsubmitted	PT7 + crtE + PT7 + crtB + PT7 + crtI + PT7 + nptIIBB	DK, BC	pSB1C3	Chloramphenicol	Geneticin
pC13DM	unsubmitted	PT7 + rbs + crtB + PT7 + rbs + crtI	CV, CW	pSB1C3	Chloramphenicol	N/A
pC13DN	unsubmitted	PT7 + rbs + crtE + PT7 + rbs + crtB + PT7 + rbs + crtI	DM, CU	pSB1C3	Chloramphenicol	N/A
pC13DO	unsubmitted	Gan. PgpdA + crtE	BI, I	pSB1C3	Chloramphenicol	N/A
pC13DP	unsubmitted	Asp. PgpdA + crtB	BG, M	pSB1C3	Chloramphenicol	N/A
pC13DQ	unsubmitted	Asp. PgpdA + crtB + PtrpC + crtI	DP, BD	pSB1C3	Chloramphenicol	N/A
pC13DR	unsubmitted	Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI	DQ, DO	pSB1C3	Chloramphenicol	N/A
pC13DS	unsubmitted	Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI + PtrpC + nptII	DS, DR	pSB1C3	Chloramphenicol	Geneticin
pC13DT	unsubmitted	T7 + crtEBI	A, D	pSB1C3	Chloramphenicol	N/A
pC13DU	unsubmitted	Lhom + PtrpC + nptIIBB + Rhom	BK, BM, Y	pSB1C3	Chloramphenicol	Geneticin
pC13DV	unsubmitted	Lhom + PgpdA + nptIIBB + Rhom	BK, BM ,BQ	pSB1C3	Chloramphenicol	Geneticin
pC13DW	unsubmitted	Lhom + PgpdA + hphBB + Rhom	BK, BM, BR	pSB1C3	Chloramphenicol	Hygromycin
pS13DX	invalid	Lhom + PtrpC + nptIIBB + Rhom	BK, BM, Y	pOSCAR	Spectinomycin	Geneticin
pS13DY	invalid	Lhom + PgpdA + nptIIBB + Rhom	BK, BM ,BQ	pOSCAR	Spectinomycin	Geneticin
pS13DZ	invalid	Lhom + PgpdA + hphBB + Rhom	BK, BM, BR	pOSCAR	Spectinomycin	Hygromycin
pAh13EA	invalid	hph + C. heterostrophus Pgpd	BK, BM, BR	pHPVEL1	Spectinomycin	Hygromycin
pS13EB	invalid	pOSCAR backbone for transformation into agrobacterium	BK, BM, BR	pOSCAR	Spectinomycin	Hygromycin

@@ Line 14: / Line 14: @@
 <table class="tableizer-table" rules="all">
 <tr class="tableizer-firstrow"><th>Plasmid Name</th><th>Part</th><th>Contents/Description</th><th>Origin</th><th>Backbone</th><th>Antibiotic Resistance</th><th>Antifungal Resistance</th></tr>
-  <tr><td>pC13A</td><td>BBa_K523022</td><td>crtEIB</td><td>Kit Plate 1 8P</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
+  <tr><td>pC13A</td><td>BBa_K523022</td><td>crtEBI</td><td>Kit Plate 1 8P</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
   <tr><td>pC13B</td><td>BBa_K539119</td><td>crtY</td><td>Kit Plate 1 12K</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
   <tr><td>pA13C</td><td>pIVEX2.3d</td><td>T7 promoter/terminator</td><td>Luo Lab</td><td>pIVEX2.3d</td><td>Ampicillin</td><td>N/A</td></tr>
@@ Line 137: / Line 137: @@
   <tr><td>pC13DR</td><td>unsubmitted</td><td>Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI</td><td>DQ, DO</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
   <tr><td>pC13DS</td><td>unsubmitted</td><td>Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI + PtrpC + nptII</td><td>DS, DR</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Geneticin</td></tr>
+`<tr><td>pC13DT</td><td>unsubmitted</td><td>T7 + crtEBI</td><td>A, D</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
+ <tr><td>pC13DU</td><td>unsubmitted</td><td>Lhom + PtrpC + nptIIBB + Rhom</td><td>BK, BM, Y</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Geneticin</td></tr>
+ <tr><td>pC13DV</td><td>unsubmitted</td><td>Lhom + PgpdA + nptIIBB + Rhom</td><td>BK, BM ,BQ</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Geneticin</td></tr>
+ <tr><td>pC13DW</td><td>unsubmitted</td><td>Lhom + PgpdA + hphBB + Rhom</td><td>BK, BM, BR</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Hygromycin</td></tr>
+ <tr><td>pS13DX</td><td>invalid</td><td>Lhom + PtrpC + nptIIBB + Rhom</td><td>BK, BM, Y</td><td>pOSCAR</td><td>Spectinomycin</td><td>Geneticin</td></tr>
+ <tr><td>pS13DY</td><td>invalid</td><td>Lhom + PgpdA + nptIIBB + Rhom</td><td>BK, BM ,BQ</td><td>pOSCAR</td><td>Spectinomycin</td><td>Geneticin</td></tr>
+ <tr><td>pS13DZ</td><td>invalid</td><td>Lhom + PgpdA + hphBB + Rhom</td><td>BK, BM, BR</td><td>pOSCAR</td><td>Spectinomycin</td><td>Hygromycin</td>
+<tr><td>pAh13EA</td><td>invalid</td><td>hph + C. heterostrophus Pgpd</td><td>BK, BM, BR</td><td>pHPVEL1</td><td>Spectinomycin</td><td>Hygromycin</td>
+<tr><td>pS13EB</td><td>invalid</td><td>pOSCAR backbone for transformation into agrobacterium</td><td>BK, BM, BR</td><td>pOSCAR</td><td>Spectinomycin</td><td>Hygromycin</td>
 </table>
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