Team:KU Leuven/Project/StickerSystem

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    <a href="https://2013.igem.org/Team:KU_Leuven/Project/Aphid_Background">
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    <h3>Aphid Background</h3>
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    <p>Crashcourse in aphid biology</p>
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    <a href="https://2013.igem.org/Team:KU_Leuven/Project/HoneydewSystem">
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    <h3>Honeydew System</h3> </a>
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    <p>Our BanAphids react to honeydew</p>
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    <a href="https://2013.igem.org/Team:KU_Leuven/Project/StickerSystem">
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    <h3>Sticker System</h3>
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    <p>You are here!</p>
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    <h3><i>E. coligy</i></h3>
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    <p>Validating the BanAphids in vivo</p>
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    <h3>Parts</h3> </a>
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    <p>BioBrick 'm all!</p>
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    <a href="https://2013.igem.org/Team:KU_Leuven/Project/DataPage">
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    <h3>Data Page</h3> </a>
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    <p>All our achievements on one small page!</p>
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Our initial system for the production of methylsalicylate and β-farnesene relied on direct interaction between the bacterium (BanAphids) and the aphid. This is ethically challenging since our BanAphids could end up in the environment. We therefore designed an alternative pheromone production system, whereby the bacteria are kept in semi-permeable pouches. These allow the pheromones to disperse in the air yet the bacteria themselves remain in the bags. In such a system bacteria have limited interactions with their environment. Consequently, honeydew would not touch the bacteria and cannot be used as a trigger, thus we have to adapt our pheromone expression system to these novel conditions. Our solution is an autonomous system. In its simplest form, this would be the constitutive expression of both pheromones. However, the constitutive production of β-farnesene rapidly renders aphids insensitive (Kunert, Reinhold and Gershenzon, 2010). The constant production of methyl-salicylate could be equally disadvantageous since ladybugs may become insensitive to the pheromone and the plant itself may end up in defence-mode too long, causing it to wilt. To prevent habituation β-farnesene en methyl-salicylate should fluctuate. This is typically achieved in an oscillator. Here we will concentrate on the modelling of a β-farnesene oscillator as a proof of principle. The production of methylsalicylate can be regulated similarly.</p>
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Our initial system for the production of methyl salicylate and β-farnesene relied on direct interaction between the bacteria and the aphids, our BanAphids would be sprayed on the plant. This is ethically and practically challenging since our <b>BanAphids</b> could end up in the environment. <b>We therefore designed an alternative pheromone production system, where the bacteria are kept in semi-permeable pouches</b>. These allow the pheromones to disperse in the air yet the bacteria themselves remain in the bags. Consequently, the presence of aphids secreting honeydew cannot be used as a trigger for the production of both pheromones. <b>Since constitutive production of β-farnesene rapidly renders aphids insensitive, concentrations should fluctuate to prevent habituation. This is typically achieved in an oscillator.</b> The oscillator is extensively elaborated in the <b><a href="https://2013.igem.org/Team:KU_Leuven/Project/Modelling/Colony_Level">oscillator model section</a></b>. Here we will mainly focus on the design of a β-farnesene oscillator, a part of it will be actually made in the lab. The production of methyl salicylate can be regulated similarly.</p>
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       <p align="justify">For those who are not afraid of having a more mathematical view on our oscillator, we invite you to read our modelling article, which can be downloaded here.</p>
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       <p align="justify">In this part we explain the necessity of the sticker system, as well as its practical execution, such as the properties of the sticker and the implementation of an oscillator. </p>
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Latest revision as of 03:19, 29 October 2013

iGem

Secret garden

Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!


  • A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
  • For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
  • We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?

Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!

tree ladybugcartoon

Aphid Background

Crashcourse in aphid biology

Honeydew System

Our BanAphids react to honeydew

Sticker System

You are here!

E. coligy

Validating the BanAphids in vivo

Parts

BioBrick 'm all!

Data Page

All our achievements on one small page!

Our initial system for the production of methyl salicylate and β-farnesene relied on direct interaction between the bacteria and the aphids, our BanAphids would be sprayed on the plant. This is ethically and practically challenging since our BanAphids could end up in the environment. We therefore designed an alternative pheromone production system, where the bacteria are kept in semi-permeable pouches. These allow the pheromones to disperse in the air yet the bacteria themselves remain in the bags. Consequently, the presence of aphids secreting honeydew cannot be used as a trigger for the production of both pheromones. Since constitutive production of β-farnesene rapidly renders aphids insensitive, concentrations should fluctuate to prevent habituation. This is typically achieved in an oscillator. The oscillator is extensively elaborated in the oscillator model section. Here we will mainly focus on the design of a β-farnesene oscillator, a part of it will be actually made in the lab. The production of methyl salicylate can be regulated similarly.

Design

In this part we explain the necessity of the sticker system, as well as its practical execution, such as the properties of the sticker and the implementation of an oscillator.

Integrating the oscillator into the wetlab

A part of the oscillator was implemented in the wetlab. You can find the genes we chose for our oscillator here, as well as the results of the wetlab experiments.

Retrieved from "http://2013.igem.org/Team:KU_Leuven/Project/StickerSystem"