Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period4/Dailylog

From 2013.igem.org

(Difference between revisions)

Latest revision as of 03:25, 29 October 2013

Small Phage September - October Notebook: October 16 - October 31 Daily Log

Small Phage

10/16/13

JL, LP

Plated for mutants as part of 10.8 Characterization of Mutant Phage.

10/18/13

JL, LP

Performed amplification and purification of insert as part of 10.18 Cloning T7 Capsid Protein into iGEM Registry.

10/19/13

JL, LP

Ran a low melt gel for 10.18 Cloning T7 Capsid Protein into iGEM Registry.

10/20/13

JL

Performed the repeat PCR and gel step in 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/21/13

JL, LP

Did restriction digest for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/22/13

JL

Low-melt gel for purification of sticky-ended insert/vector for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/23/13

JL, LP

Did ligation and transformation for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/24/13

JL, LP

Did Colony PCR and Overnights for Vector Purification for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/25/13

JL, LP

Did plasmid cleanup for 10.18 Cloning T7 Capsid Protein into iGEM Registry and sent the plasmid into iGEM!

10/27/13

JL

Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for 10.8 Characterization of Mutant Phage.

10/28/13

JL, LP

Propagated WT, S4, S10, S21, S22, and L8 for 10.8 Characterization of Mutant Phage

Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for 10.8 Characterization of Mutant Phage.

Mapped out mutation for S4 capsid protein.

Finished the wiki.

@@ Line 66: / Line 66: @@
 <br>
-<font size="4"> '''10/9/13''' </font>
+<font size="4"> '''10/21/13''' </font>
 JL, LP
-* Purified the propagated mutant from [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
+* Did restriction digest for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
-* Started approximately 20 mL of E coli B liquid culture overnight.
 <br>
-<font size="4"> '''10/10/13''' </font>
+<font size="4"> '''10/22/13''' </font>
-LP
+JL
-* Performed spot tests to estimate the mutant phage titer in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
+* Low-melt gel for purification of sticky-ended insert/vector for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
-* Started approximately 20 mL of E coli B liquid culture overnight.
+<br>
-JL
+<font size="4"> '''10/23/13''' </font>
-* Designed primers for cloning the major and minor capsid proteins into the iGEM registry: BI309 (Xbal) and BI310 (SpeI)
+JL, LP
+* Did ligation and transformation for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
 <br>
-<font size="4"> '''10/11/13''' </font>
+<font size="4"> '''10/24/13''' </font>
 JL, LP
-* Plated the mutant phage at -7 to verify their phenotypic stability. For specifics, please see [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
+* Did Colony PCR and Overnights for Vector Purification for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
-* Designed primers for more accurate sequencing: BI319 (160bp upstream from start codon) and BI320 (-160 downstream from minor capsid protein stop codon)
 <br>
-<font size="4"> '''10/12/13''' </font>
+<font size="4"> '''10/25/13''' </font>
-JL
+JL, LP
-* Started approximately 15mL of E coli B liquid culture overnight
+* Did plasmid cleanup for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]] and sent the plasmid into iGEM!
 <br>
-<font size="4"> '''10/13/13''' </font>
+<font size="4"> '''10/27/13''' </font>
 JL
-* Plated more phage from CsCl gradient to select for mutant. Specifics are recorded in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
+* Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
 <br>
-<font size="4"> '''9/27/13''' </font>
+<font size="4"> '''10/28/13''' </font>
 JL, LP
-* Determined results for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
+* Propagated WT, S4, S10, S21, S22, and L8 for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]]
-* Finished updating the wiki.
+* Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
-* Started approximately 30mL of E. coli B overnight.
+* Mapped out mutation for S4 capsid protein.
+* Finished the wiki.
+<br>
 <br>