Team:Cornell/notebook/protocols

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{{:Team:Cornell/templates/header}}
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<script type="text/javascript">
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$(window).load(function() {
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$('li.pg-notebook_protocols').addClass('active');
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<div class="row main-image">
<div class="row main-image">
<div class="twelve columns">
<div class="twelve columns">
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<img src="https://static.igem.org/mediawiki/2012/9/93/Swati_and_Claire.jpg">
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<img src="https://static.igem.org/mediawiki/2013/6/60/Cornell_IMG_4991_small.jpg" style="width:940px; height:450px">
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<a class="button radius gallery-button" href="https://2012.igem.org/Team:Cornell/project/gallery">More Photos</a>
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<a class="button radius gallery-button" href="https://2013.igem.org/Team:Cornell/team/gallery">More Photos</a>
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<li>
<li>
<a href="https://static.igem.org/mediawiki/2012/2/2d/Qiagen_Miniprep.pdf" target="_blank">Qiagen Miniprep</a>
<a href="https://static.igem.org/mediawiki/2012/2/2d/Qiagen_Miniprep.pdf" target="_blank">Qiagen Miniprep</a>
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</li>
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<li>
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<a href="https://static.igem.org/mediawiki/2013/7/79/Column_Purification.pdf" target="_blank">Column Purification</a>
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</li>
</ul>
</ul>
</div>
</div>
<div class="four columns">
<div class="four columns">
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<h4>DNA Assessment</h4>
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<h4>Plasmid Editing</h4>
<ul>
<ul>
<li>
<li>
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<a href="https://static.igem.org/mediawiki/2012/6/6c/DNA_Gel_Electrophoresis.pdf" target="_blank">DNA Gel Electrophoresis</a>
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<a href="https://static.igem.org/mediawiki/2012/a/af/Double_Digest.pdf" target="_blank">Double Digest</a>
</li>
</li>
<li>
<li>
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<a href="https://static.igem.org/mediawiki/2012/0/03/Sequencing_Preparation.pdf" target="_blank">Preparation of DNA for Sequencing</a>
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<a href="https://static.igem.org/mediawiki/2012/f/fb/Single_Digest.pdf" target="_blank">Single Digest</a>
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</li>
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<li>
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<a href="https://static.igem.org/mediawiki/2012/a/a5/Site_Directed_Mutagenesis.pdf" target="_blank">Site Directed Mutagenesis</a>
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</li>
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<li>
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<a href="https://static.igem.org/mediawiki/2012/a/a7/T4_Ligase_Ligation.pdf" target="_blank">T4 Ligase Ligation</a>
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</li>
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<a href="https://static.igem.org/mediawiki/2013/9/94/Gibson_Assembly.pdf" target="_blank">Gibson Assembly</a>
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<h4>PCR Methods</h4>
<h4>PCR Methods</h4>
<ul>
<ul>
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<a href="https://static.igem.org/mediawiki/2013/3/30/Q5_PCR.pdf" target="_blank">Q5 PCR</a>
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</li>
<li>
<li>
<a href="https://static.igem.org/mediawiki/2012/2/26/Phusion_PCR.pdf" target="_blank">Phusion PCR</a>
<a href="https://static.igem.org/mediawiki/2012/2/26/Phusion_PCR.pdf" target="_blank">Phusion PCR</a>
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<li>
<li>
<a href="https://static.igem.org/mediawiki/2012/5/57/Making_Ecoli_Electrocompetent.pdf" target="_blank">Preparing Electrocompetent <i>E.coli</i></a>
<a href="https://static.igem.org/mediawiki/2012/5/57/Making_Ecoli_Electrocompetent.pdf" target="_blank">Preparing Electrocompetent <i>E.coli</i></a>
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</li>
 
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<a href="https://static.igem.org/mediawiki/2012/6/6f/MyersMyers_Shew-E-poration.pdf" target="_blank">Myers and Myers Electoporation of <i>Shewanella</i></a>
 
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<a href="https://static.igem.org/mediawiki/2012/f/f7/PNNL_Electrocompetent_Shewanella.pdf" target="_blank">PNNL’s Preparing Electrocompetent <i>Shewanella</i></a>
 
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<li>
 
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<a href="https://static.igem.org/mediawiki/2012/0/0f/Shewanella_Conjugation.pdf" target="_blank">Conjugation of Plasmids into <i>Shewanella</i> </a>
 
</li>
</li>
</ul>
</ul>
</div>
</div>
<div class="four columns">
<div class="four columns">
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<h4>Plasmid Editing</h4>
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<h4>DNA Assessment</h4>
<ul>
<ul>
<li>
<li>
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<a href="https://static.igem.org/mediawiki/2012/a/af/Double_Digest.pdf" target="_blank">Double Digest</a>
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<a href="https://static.igem.org/mediawiki/2012/6/6c/DNA_Gel_Electrophoresis.pdf" target="_blank">DNA Gel Electrophoresis</a>
</li>
</li>
<li>
<li>
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<a href="https://static.igem.org/mediawiki/2012/f/fb/Single_Digest.pdf" target="_blank">Single Digest</a>
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<a href="https://static.igem.org/mediawiki/2012/0/03/Sequencing_Preparation.pdf" target="_blank">Preparation of DNA for Sequencing</a>
</li>
</li>
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<li>
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                                        <li>
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<a href="https://static.igem.org/mediawiki/2012/a/a5/Site_Directed_Mutagenesis.pdf" target="_blank">Site Directed Mutagenesis</a>
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<a href="https://static.igem.org/mediawiki/2013/5/52/Fluorescence_Assay_Procedure.pdf" target="_blank">Fluorescence Assay</a>
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<li>
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<a href="https://static.igem.org/mediawiki/2012/a/a7/T4_Ligase_Ligation.pdf" target="_blank">T4 Ligase Ligation</a>
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</li>
</li>
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</ul>
</ul>
</div>
</div>
<div class="four columns">
<div class="four columns">
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<h4><i>Shewanella</i> Growth</h4>
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<h4>Fungal Transformation</h4>
<ul>
<ul>
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<li>
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<a href="https://static.igem.org/mediawiki/2012/6/6c/Growth_Assay_Protocol.pdf" target="_blank"><i>Shewanella</i> Growth Assay Protocol</a>
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<a href="https://static.igem.org/mediawiki/2013/5/50/Preparation_of_Protoplasts.pdf" target="_blank">Preparation of Protoplasts</a>
</li>
</li>
<li>
<li>
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<a href="https://static.igem.org/mediawiki/2012/b/bc/M4_recipe.pdf" target="_blank">M4 Recipe</a>
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<a href="https://static.igem.org/mediawiki/2013/f/f0/Transformation_of_Protoplasts.pdf" target="_blank">Transformation of Protoplasts</a>
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<a href="https://static.igem.org/mediawiki/2012/c/cb/Synthetic_River_Media.pdf" target="_blank">Synthetic River Media Recipe</a>
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<a href="https://static.igem.org/mediawiki/2013/9/91/Agrobacteria_Mediated_Transformation_Protocol.pdf " target="_blank">Agrobacteria Mediated Transformation</a>
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<li>
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<a href="https://2012.igem.org/Team:Cornell/protocols/secret_dylan_dance" target="_blank">Plate Drying</a>
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</li>
</li>
</ul>
</ul>
</div>
</div>
</div>
</div>
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Latest revision as of 03:44, 29 October 2013

Cornell University Genetically Engineered Machines

More Photos

DNA Purification

Plasmid Editing

PCR Methods

Plasmid Insertion

DNA Assessment

Fungal Transformation

Retrieved from "http://2013.igem.org/Team:Cornell/notebook/protocols"