Team:BostonU/Protocols

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<h1>Prtocols</h1>
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<li><a href="#">Our Team</a>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Team">Team</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Social">Summer Fun</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Project_Overview">Project Overview and Abstract</a></li>
 
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                        <li><a href="https://2013.igem.org/Team:BostonU/MoCloChara">Introduction to MoClo and Characterization</a></li>
 
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                            <li><a href="https://2013.igem.org/Team:BostonU/QS">Quorum Sensing</a></li>
 
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                            <li><a href="https://2013.igem.org/Team:BostonU/HK">Histidine Kinase</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Results ">Results Summary</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Data">Data Collected</a></li>
 
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<li><a href="#">Notebook</a>
 
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<ul>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Protocols">Protocols</a></li>
 
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                        <li><a href="https://2013.igem.org/Team:BostonU/NotebookML">Characterization Notebook</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/NotebookQS">Quorum Sensing Notebook</a></li>
 
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                        <li><a href="https://2013.igem.org/Team:BostonU/NotebookHK">Histidine Kinase Notebook</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Clotho">Clotho Notebook</a></li>
 
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        <li><a href="https://2013.igem.org/Team:BostonU/PUP">Puppeteer Notebook</a></li>
 
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                        <li><a href="https://2013.igem.org/Team:BostonU/TroubleShooting">Troubleshooting</a></li>
 
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<li><a href="#">Considerations</a>
 
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<li><a href="https://2013.igem.org/Team:BostonU/NEGEM">New England iGEM Regional Meeting</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Human Practices">Human Practices</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Safety">Safety</a></li>
 
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<li><a href="#">Acknowledgements</a>
 
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                    <li><a href="https://2013.igem.org/Team:BostonU/Collaborations">Collaborations</a></li>
 
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                    <li><a href="https://2013.igem.org/Team:BostonU/Acknowledgements">Acknowledgements</a></li>
 
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<h4>Protocols</h4>
 
<h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook.</p>
<h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook.</p>
We hope they help anyone interested in our experiments to successfully repeat them.
We hope they help anyone interested in our experiments to successfully repeat them.

Revision as of 14:14, 19 July 2013



Prtocols

This section contains the protocols that we used during the summer and that are mentioned in our Notebook.

We hope they help anyone interested in our experiments to successfully repeat them.


  • DNA Quantification

  • Gel Extraction

  • Miniprep

  • PCR Cleanup

  • Ligation

  • Media Prep

  • MoClo Level 0 Reaction

  • MoClo Level 1 and 2 Reactions

  • Phusion PCR

  • Restriction Digest

  • Restriction Digest 10 µL mapping

  • Transformation

    Retrieved from "http://2013.igem.org/Team:BostonU/Protocols"