"
Team:Groningen/Labwork/19 July 2013
From 2013.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
<br> No colonies have grown on the cm plates. | <br> No colonies have grown on the cm plates. | ||
<br> There were supposed to be colonies that contained the GFP biobrick. | <br> There were supposed to be colonies that contained the GFP biobrick. | ||
| + | |||
| + | |||
| + | '''Sander and Erik-Jan''' | ||
| + | <br> Did a PCR of Silk 2 (strepF-R) and Silk 3 (F-StrepR). | ||
| + | <br> annealing temperatures of 78 C for silk 2 an 80 C for silk 3 | ||
| + | |||
| + | '''Sander''' | ||
| + | <br> ran restriction analysis of silk 1 (F-R) on 0,8% agarose gel. 90 V for 34 min. | ||
| + | <br> low concentration on gel. | ||
| + | <br> did a ligation reaction on silk 1 with each of the Signal Sequences (MotB, FliZ, EstA and LytB) at 1:1 ratio. | ||
| + | <br> did a PCR of ligation reactions. same protocol as silk 3 above. | ||
Revision as of 13:51, 20 July 2013
Inne
No colonies have grown on the cm plates.
There were supposed to be colonies that contained the GFP biobrick.
Sander and Erik-Jan
Did a PCR of Silk 2 (strepF-R) and Silk 3 (F-StrepR).
annealing temperatures of 78 C for silk 2 an 80 C for silk 3
Sander
ran restriction analysis of silk 1 (F-R) on 0,8% agarose gel. 90 V for 34 min.
low concentration on gel.
did a ligation reaction on silk 1 with each of the Signal Sequences (MotB, FliZ, EstA and LytB) at 1:1 ratio.
did a PCR of ligation reactions. same protocol as silk 3 above.
