Exeter/19 July 2013

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(Difference between revisions)
(Created page with "==Transformations== We are transforming... *New Part 1 - CcaS (green light sensor) + terminator *New Part 2 - (promoter, RBS and FixJ) + terminator *New Part 3 - Yellow pigment ...")
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==(Monday morning, results of transformation)==
==(Monday morning, results of transformation)==
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FILL THIS IN, FRAN!
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{| class="wikitable"
 +
|-
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! Part !! Number of colonies
 +
|-
 +
| Different version of RBS + cph8 || 23
 +
|-
 +
| OmpR || 120
 +
|-
 +
| Lambda repressor system || 20 countable, other colonies visible but too small to numerate
 +
|-
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| RFP control || 75
 +
|-
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| New Part 1 || 78
 +
|-
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| New Part 2 || 2
 +
|-
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| New Part 3 || 12
 +
|-
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| New Part 4 || '''0'''
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|-
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| New Part 7 || 6
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|-
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| New Part 8 || 1
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|-
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| Ligation Control || colonies present, too small to count, but approx. 100
 +
|}

Revision as of 10:15, 22 July 2013

Transformations

We are transforming...

  • New Part 1 - CcaS (green light sensor) + terminator
  • New Part 2 - (promoter, RBS and FixJ) + terminator
  • New Part 3 - Yellow pigment + terminator
  • New Part 4 - Magenta pigment + terminator
  • New Part 7 - YF1 (blue light sensor) + terminator
  • New Part 8 - FixJ + terminator
  • Ligation control (RFP)
  • Retry of OmpR (BBa_K098011) from Kit Plate (failed on 16/7/13)
  • Retry of lambda repressor system (BBa_Q04510) from Kit Plate (failed on 16/7/13)
  • Different version of RBS + cph8 (red light sensor) from Kit Plate

(Monday morning, results of transformation)

Part Number of colonies
Different version of RBS + cph8 23
OmpR 120
Lambda repressor system 20 countable, other colonies visible but too small to numerate
RFP control 75
New Part 1 78
New Part 2 2
New Part 3 12
New Part 4 0
New Part 7 6
New Part 8 1
Ligation Control colonies present, too small to count, but approx. 100
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