Template:Team:Bielefeld-Germany/Notebook/Week02

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<div class="article" id="w02-13-05">
 
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<p><a name="week02-13-05">Week 2, 13.05.2013, Monday</a></p>
 
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<p>Posted on 10/06/2013</p>
 
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                <p>
 
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<b>1) Experiment 1</b><br/>
 
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We did it!<br/><br/>
 
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<b>2) Experiment 2</b><br/>
 
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                Succeeded!
 
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<p><a name="week02-14-05">Week 2, 14.05.2013, Tuesday</a></p>
 
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<p>Posted on 10/06/2013</p>
 
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<b>1) Starting with experiment Glycerol dehydrogenase </b><br/>
 
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                - Preparing overnight culture ''E. coli'' KRX <br/><br/>
 
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<b>2) Experiment 2 - Incubation</b><br/>
 
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Description of experiment 2
 
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<p><a name="week01-15-05">Week 2, 15.05.2013, Wednesday</a></p>
 
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<div class="article-details">
 
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<p>Posted on 10/06/2013</p>
 
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<b>1) Glycerol dehydrogenase </b><br />
 
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- Using overnight culture ''E. coli'' KRX <br />
 
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                - Genome Isolation ''E. coli'' KRX using innuPrep Plasmid MiniKit ''JenaAnalytik'' <br />
 
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                - Using ''E. coli'' KRX Genome DNA for PCR <br />
 
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                - PCR-program: <br />
 
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|H2O
 
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|5 x Phusion CC Buffer
 
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|10 μL
 
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|10 mM dNTPs
 
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|2 μL
 
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|Fwd and Rev Primer Mix 10 μM
 
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|Tempalte 100 ng
 
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                Table PCR-program <br />
 
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                - PCR products are used for agarose gel electrophoresis <br />
 
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                Table gel-program <br />
 
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                - Agarose gel electrophoresis failed, no bands found <br />
 
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<br /><br />
 
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<b>2) Experiment 2</b><br />
 
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<p><a name="week01-09-05">Week 1, 09.05.2013, Thursday</a></p>
 
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<p>Posted on 10/06/2013</p>
 
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<p>
 
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<b>1) Glycerol dehydrogenase </b><br />
 
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- Using'' E. coli'' KRX Genome DNA for PCR <br />
 
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                - PCR-program: <br />
 
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                Table PCR-components <br />
 
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                Table PCR-program <br />
 
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                - PCR products are used for agarose gel electrophoresis <br />
 
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                Table gel-program <br />
 
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                - Agarose gel electrophoresis worked, see image. <br />
 
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                Picture Agarosegel glda_1 <br />
 
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                - Bands are at expected size of 1050 bp <br />
 
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<br /><br />
 
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<b>2) Experiment 2</b><br />
 
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<p><a name="week01-10-05">Week 1, 10.05.2013, Friday</a></p>
 
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<p>Posted on 10/06/2013</p>
 
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<b> 1) Glycerol dehydrogenase </b> <br />
 
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- Repeating agarose gel electrophoresis form 23.05.2013 with more sample volume <br />
 
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                - Isolation of bands at 1050 bp using innuPrep Gel Extraction Kit ''JenaAnalytik'' <br />
 
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                - Measurement of DNA-concentration using nanodrop <br />
 
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Latest revision as of 14:04, 22 July 2013