Team:BostonU/Protocols

From 2013.igem.org

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<h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook.</p>
<h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook.</p>
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We hope they help anyone interested in our experiments to successfully repeat them.
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We hope they help anyone interested in our experiments to successfully repeat them.\
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</h7>
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<li><a href="https://static.igem.org/mediawiki/2013/7/7e/NanoDrop_DNA_Quantification.xls">DNA Quantification</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/7/7e/NanoDrop_DNA_Quantification.xls" style="color:#B22222; display:inline;background-color:white">DNA Quantification</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/2/2c/Epoch_Gel_extraction.xls">Gel Extraction</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/3/3b/Epoch_Miniprep.xls">Miniprep</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/2/2c/Epoch_Gel_extraction.xls" style="color:#B22222; display:inline;background-color:white">Gel Extraction</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/6/6e/Epoch_PCR_cleanup.xls">PCR Cleanup</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/0/01/Ligations.xls">Ligation</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/3/3b/Epoch_Miniprep.xls" style="color:#B22222; display:inline;background-color:white">Miniprep</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/b/bd/Media_Preparation_LB_broth_agar_Gels.xls">Media Prep </a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/6/61/MoClo_Level_0.xls">MoClo Level 0 Reaction</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/6/6e/Epoch_PCR_cleanup.xls" style="color:#B22222; display:inline;background-color:white">PCR Cleanup</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/4/4d/MoClo_Level_1_and_2.xls">MoClo Level 1 and 2 Reactions</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/d/dc/Phusion_PCR.xls">Phusion PCR</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/0/01/Ligations.xls" style="color:#B22222; display:inline;background-color:white">Ligation</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/0/05/Restriction_Digest.xls">Restriction Digest</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/8/8e/Restriction_Digest_mapping_10uLs.xls">Restriction Digest 10 &#181;L mapping</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/b/bd/Media_Preparation_LB_broth_agar_Gels.xls" style="color:#B22222; display:inline;background-color:white">Media Prep </a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/8/8b/Transformation.xls">Transformation</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/6/61/MoClo_Level_0.xls" style="color:#B22222; display:inline;background-color:white">MoClo Level 0 Reaction</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/4/4d/MoClo_Level_1_and_2.xls" style="color:#B22222; display:inline;background-color:white">MoClo Level 1 and 2 Reactions</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/d/dc/Phusion_PCR.xls" style="color:#B22222; display:inline;background-color:white">Phusion PCR</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/0/05/Restriction_Digest.xls" style="color:#B22222; display:inline;background-color:white">Restriction Digest</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/8/8e/Restriction_Digest_mapping_10uLs.xls" style="color:#B22222; display:inline;background-color:white">Restriction Digest 10 &#181;L mapping</a></p>
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<li><a href="https://static.igem.org/mediawiki/2013/8/8b/Transformation.xls" style="color:#B22222; display:inline;background-color:white">Transformation</a></p>
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Revision as of 14:21, 22 July 2013



Protocols

This section contains the protocols that we used during the summer and that are mentioned in our Notebook.

We hope they help anyone interested in our experiments to successfully repeat them.\



  • DNA Quantification

  • Gel Extraction

  • Miniprep

  • PCR Cleanup

  • Ligation

  • Media Prep

  • MoClo Level 0 Reaction

  • MoClo Level 1 and 2 Reactions

  • Phusion PCR

  • Restriction Digest

  • Restriction Digest 10 µL mapping

  • Transformation