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Team:BostonU/NotebookML
From 2013.igem.org
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</ul> | </ul> | ||
</ul> | </ul> | ||
| + | <li>Assemble level 1 circuits for characterization of Constitutive Promoter Library</li> | ||
| + | <ul> | ||
| + | <li>160 circuits that have different combinations of J231XX promoters and RBS’s</li> | ||
| + | </ul> | ||
| + | <ul> | ||
| + | <li>1 set with all promoters + BCD2</li> | ||
| + | <li>1 set with all RBS’s+J23100</li> | ||
| + | </ul> | ||
| + | <li>Assemble level 1 circuits for characterization of Repressible Promoter</li> | ||
| + | <li>Run flow cytometry on the promoter characterization circuits</li> | ||
| + | <li>Assemble level 2 inverter as an example of the library’s use</li> | ||
| + | <li>Switch the backbone in some existing level 1 parts to study how different antibiotics affect the flow cytometry data: <strong>6/21/13<strong></li> | ||
</ul> | </ul> | ||
Revision as of 03:41, 23 July 2013
Characterization Notebook
- Assemble level 0 parts needed
- Constitutive Promoter Characterization Project:
- J23100_AB, J23113_AB, J23115_AB, J23115_EB, J23116_AB, J23116_EB, J23117_AB, J23118_AB, J23119_AB
- BCD1_BC, BCD2_BC, BCD8_BC, BCD12_BC, BCD13_BC, BCD16_BC: completed 6/27/13
- Repressible Promoter Characterization Project:
- C0012 (LacI), C0062_CD (LuxR), C0071_CD (rhlR_CD), pLacI (R0010_FB)
- Assemble level 1 circuits for characterization of Constitutive Promoter Library
- 160 circuits that have different combinations of J231XX promoters and RBS’s
- 1 set with all promoters + BCD2
- 1 set with all RBS’s+J23100
- Assemble level 1 circuits for characterization of Repressible Promoter
- Run flow cytometry on the promoter characterization circuits
- Assemble level 2 inverter as an example of the library’s use
- Switch the backbone in some existing level 1 parts to study how different antibiotics affect the flow cytometry data: 6/21/13
