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Team:Groningen/Labwork/25 June 2013
From 2013.igem.org
(Difference between revisions)
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<h2>Mirjam, Chaline and Inne</h2> | <h2>Mirjam, Chaline and Inne</h2> | ||
| - | + | A couple of media are prepared: | |
| - | <br/>LB medium with agar 1 liter | + | <br/>-LB medium with agar 1 liter |
| - | <br/>LB medium divided into 80 ml portions | + | <br/>-LB medium 0.5 liter divided into 60/80 ml portions |
| - | <br/>70% ethanol | + | <br/>-70% ethanol |
| - | <br/> | + | <br/>-1x TBE buffer 2 liter |
| - | Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 | + | <br>Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving, the LB medium with agar is placed in the 55°C stove. |
<h2>Mirjam and Claudio</h2> | <h2>Mirjam and Claudio</h2> | ||
| - | <br/>''B. subtilis'' strain 168 is inoculated O/N at 37 | + | <br/>''B. subtilis'' strain 168 is inoculated O/N at 37°C and every buffer necessary for the extraction of genomic DNA is arranged. |
<h2>Claudio</h2> | <h2>Claudio</h2> | ||
| - | + | Continued the design for the heat motility. | |
| - | + | ||
Revision as of 15:23, 28 July 2013
Mirjam, Chaline and Inne
A couple of media are prepared:-LB medium with agar 1 liter
-LB medium 0.5 liter divided into 60/80 ml portions
-70% ethanol
-1x TBE buffer 2 liter
Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving, the LB medium with agar is placed in the 55°C stove.
Mirjam and Claudio
''B. subtilis'' strain 168 is inoculated O/N at 37°C and every buffer necessary for the extraction of genomic DNA is arranged.
