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Team:Groningen/Labwork/25 June 2013
From 2013.igem.org
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<h2>Mirjam, Chaline and Inne</h2> | <h2>Mirjam, Chaline and Inne</h2> | ||
| - | + | A couple of media are prepared: | |
| - | <br/>LB medium with agar 1 liter | + | <br/>-LB medium with agar 1 liter |
| - | <br/>LB medium divided into 80 ml portions | + | <br/>-LB medium 0.5 liter divided into 60/80 ml portions |
| - | <br/>70% ethanol | + | <br/>-70% ethanol |
| - | <br/> | + | <br/>-1x TBE buffer 2 liter |
| - | Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 | + | <br>Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving, the LB medium with agar is placed in the 55°C stove. |
| - | + | <p> | |
| - | < | + | <h2>Mirjam and Claudio</h2> |
| + | <i>B. subtilis</i> strain 168 is inoculated in LB medium O/N at 37°C and every buffer necessary for the <a href="https://2013.igem.org/Team:Groningen/protocols/Genomic_DNA_extraction"><FONT COLOR="black"><b>genomic DNA extraction</b></FONT></a> is arranged. | ||
| + | |||
| + | <p> | ||
| + | <h2>Claudio</h2> | ||
| + | Continued the design for the heat motility. | ||
Latest revision as of 09:18, 30 July 2013
Mirjam, Chaline and Inne
A couple of media are prepared:-LB medium with agar 1 liter
-LB medium 0.5 liter divided into 60/80 ml portions
-70% ethanol
-1x TBE buffer 2 liter
Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving, the LB medium with agar is placed in the 55°C stove.
Mirjam and Claudio
B. subtilis strain 168 is inoculated in LB medium O/N at 37°C and every buffer necessary for the genomic DNA extraction is arranged.
