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06/08/13
From 2013.igem.org
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*expectation-7kb band, confirming the presence of the limonene biobrick (5kb) and chloramphenicol backbone (2kb) | *expectation-7kb band, confirming the presence of the limonene biobrick (5kb) and chloramphenicol backbone (2kb) | ||
[[File:igem_single_dig_060813.jpg]] | [[File:igem_single_dig_060813.jpg]] | ||
| + | *The gel shows the presence of 7kb bands for both digests of samples 10.1, 10.2 and the 5.1 sample, which was expected. | ||
| + | *No bands appeared for samples 5.1. | ||
| + | *For samples 10.3 there is a 2kb band, indicating that with the ligation, the chloroamphenicol circulized. | ||
==Making chloroamphenicol agar plates== | ==Making chloroamphenicol agar plates== | ||
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**Pour out onto 20 petri dishes | **Pour out onto 20 petri dishes | ||
**Leave to set on bench | **Leave to set on bench | ||
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| + | ==Plating out samples== | ||
| + | *Plating out samples 10.1, 10.2, 10.3 and 5.1 onto chloroamphenicol plates. | ||
| + | *Grow overnight at 37C | ||
Latest revision as of 14:31, 6 August 2013
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Running of an agarose gel
- Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
- expectation-7kb band, confirming the presence of the limonene biobrick (5kb) and chloramphenicol backbone (2kb)
- The gel shows the presence of 7kb bands for both digests of samples 10.1, 10.2 and the 5.1 sample, which was expected.
- No bands appeared for samples 5.1.
- For samples 10.3 there is a 2kb band, indicating that with the ligation, the chloroamphenicol circulized.
Making chloroamphenicol agar plates
- Making additional plates
- 400ml of agar
- Melt in microwave
- 3mins on low -> shake
- Repeat
- 2mins on low -> shake
- Repeat
- Add 800ul of chloroamphenicol at a concentration of 25 ul/ml
- Pour out onto 20 petri dishes
- Leave to set on bench
- 400ml of agar
Plating out samples
- Plating out samples 10.1, 10.2, 10.3 and 5.1 onto chloroamphenicol plates.
- Grow overnight at 37C
