Our project has two major goals: 1. To introduce MoClo as an alternative assembly method for use by iGEM teams and 2. To develop a standard protocol for the characterization of genetic circuits containing fluorescent proteins and share this protocol with the synthetic biology community.
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In order to achieve these goals, there many steps must be taken. First, we must convert BioBrick Parts into MoClo Parts using PCR. Upon converting basic parts, our project will have three thrusts:
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<li><a href="https://2013.igem.org/Team:BostonU/MoClo2">Building:</a> Build Genetic Circuits with MoClo Parts
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<li><a href="https://2013.igem.org/Team:BostonU/Characterization">Characterizing:</a> Characterize Circuits using Flow Cytometry
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<li><a href="https://2013.igem.org/Team:BostonU/DataSheet">Sharing:</a> Generate Data Sheet for MoClo Parts</ul>
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As we worked towards our first goal over the summer months, we ran into difficulties with some of our PCR and cloning reactions. This has unfortunately delayed our other goals, but we're still working hard towards generating a characterization workflow and a MoClo data sheet format for the Jamboree.
¹iGEM Team Member, ²iGEM Team Mentor, ³iGEM Team Advisor, §Faculty Sponsor, Department of Electrical and Computer Engineering, Boston University, Boston, MA, USA
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