Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.6 T7+ Liquid Culture Phage Concentration Test
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'''III) Reagants Used''' | '''III) Reagants Used''' | ||
- | : E. Coli BL21 overnight from 5.5; T7+ from 5.3 Phage | + | : E. Coli BL21 overnight from 5.5; T7+ from [[5.3 Phage amplification/purification test]]; LB from 4.5 |
'''IV) Actual Procedure''' | '''IV) Actual Procedure''' | ||
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: - Liquid culture was incubated for approximately 48 hours (5.6 4:00pm - 5.8 6:00pm) | : - Liquid culture was incubated for approximately 48 hours (5.6 4:00pm - 5.8 6:00pm) | ||
Spot test to test amplification results (5.8) | Spot test to test amplification results (5.8) | ||
- | : - Phage liquid culture was purified and spot test was performed using procedures similar to those in 5.3 Phage | + | : - Phage liquid culture was purified and spot test was performed using procedures similar to those in [[5.3 Phage amplification/purification]] |
: - The purified stock was labeled 5.6 | : - The purified stock was labeled 5.6 | ||
: - Spot test plates were incubated overnight | : - Spot test plates were incubated overnight |
Revision as of 22:14, 21 May 2013
5.6 T7+ Liquid Culture Phage Concentration Test
I) Purpose
- To determine if the volume of phage stock added effects the liquid culture concentration result
II) Expected Outcome
- We hope that 100uL of phage will be enough to increase the liquid culture concentration
III) Reagants Used
- E. Coli BL21 overnight from 5.5; T7+ from 5.3 Phage amplification/purification test; LB from 4.5
IV) Actual Procedure
Liquid culture set up (5.6)
- - Three test tubes were set up. To each, add 4mL of LB and 1mL of E coli BL21 overnight
- - Add 10uL, 100uL, and 1mL to each tube, respectively.
- - Liquid culture was incubated for approximately 48 hours (5.6 4:00pm - 5.8 6:00pm)
Spot test to test amplification results (5.8)
- - Phage liquid culture was purified and spot test was performed using procedures similar to those in 5.3 Phage amplification/purification
- - The purified stock was labeled 5.6
- - Spot test plates were incubated overnight
V) Results (5.9)
- - The 10 ul spot test went down to -9, 100 ul to -8, and 1 ml to -7
- - Thus, 10 ul yields the highest titer