"
Team:MSOE Milwaukee/Modeling
From 2013.igem.org
Vanhandelp (Talk | contribs) |
Vanhandelp (Talk | contribs) |
||
| Line 8: | Line 8: | ||
After setting up a system using MATLAB (C) the final turnover rate was calculated based on the initial concentration of substrate and the inhibitors present in the system from different steps. We made the assumption that the system would be in a reactor at steady state and the following graph shows how the concentration of product will change based off of the concentration of substrate present at the inlet.<br> | After setting up a system using MATLAB (C) the final turnover rate was calculated based on the initial concentration of substrate and the inhibitors present in the system from different steps. We made the assumption that the system would be in a reactor at steady state and the following graph shows how the concentration of product will change based off of the concentration of substrate present at the inlet.<br> | ||
</P> | </P> | ||
| - | <img align = center src="https://static.igem.org/mediawiki/2013/f/fb/Graph.JPG"></img> | + | <img align = center src="https://static.igem.org/mediawiki/2013/f/fb/Graph.JPG"></img><br> |
| + | <p> | ||
| + | When the concentration of the substrate is too low we see almost no product as seen in the beginning of the graph. When a threshold is hit, the product is seen to begin increasing rapidly. The final stage shows a linear increase in the amount of product with respect to the inlet substrate concentration. | ||
</HTML> | </HTML> | ||
Revision as of 15:54, 14 August 2013
|
|
|
|
|
|
|
|
|
We chose to model the second bacteria in our system. Each enzyme was carefully researched to find experimental evidence of both the km and the vmax values. The following table shows the enzyme and the kinetic equation driving the turnover.
((Insert table here))
After setting up a system using MATLAB (C) the final turnover rate was calculated based on the initial concentration of substrate and the inhibitors present in the system from different steps. We made the assumption that the system would be in a reactor at steady state and the following graph shows how the concentration of product will change based off of the concentration of substrate present at the inlet.
When the concentration of the substrate is too low we see almost no product as seen in the beginning of the graph. When a threshold is hit, the product is seen to begin increasing rapidly. The final stage shows a linear increase in the amount of product with respect to the inlet substrate concentration.
