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| - | ==May==
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| - | ===5/1/13===
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| - | - Commencement of spring!!!
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| - | - Discussed goals and outlined plans for spring term
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| - | ===5/2/13===
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| - | - Designed primers for amplifying and sequencing phage capsid protein
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| - | - Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
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| - | ===5/3/13===
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| - | - Performed agar test, focusing primarily on ×8
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| - | : [[5.3 T7 phage selection method test]]
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| - | - Processed phage amplification
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| - | : [[5.3 T7 phage amplification/purification]]
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| - | ===5/4/13===
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| - | - Performed dilution series using stock 5.3 (-1 through -11)
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| - | - Started two 5mL overnights of BL21
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| - | ===5/5/13===
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| - | - Spot test using stock 5.3 and its dilution series (from 5.4)
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| - | : [[5.3 T7 phage amplification/purification]]
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| - | - Started liquid culture for purification team (at around noon)
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| - | : 1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)
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| - | - Started 5.5 amplification from a plaque test
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| - | : [[5.5 Amplification from a plaque test]]
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| - | ===5/6/13===
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| - | - Continued [[5.3 T7 phage amplification/purification]]
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| - | - Performed liquid culture phage concentration test
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| - | : [[5.6 T7+ Liquid Culture Phage Concentration Test]]
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| - | ===5/7/13===
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| - | - Started two 5mL of E coli BL21 overnight
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| - | - Designed procedure for applying mutagen and selecting for T7
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| - |
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| - | ===5/8/13===
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| - | - Went over procedure for applying mutagen and PCR with Dr. Grose
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| - | - Performed spot tests under [[5.6 T7+ Liquid Culture Phage Concentration Test]]
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| - | - Started two 5mL BL21 overnights
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| - | - Learned how to create LB plates
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| - |
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| - | ===5/9/13===
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| - | - Practiced with ×6 and ×8 top agar
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| - | : [[5.9 T7 selection method test #2]]
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| - | - Started [[5.9 T7+ Liquid Culture Phage Concentration Test #2]]
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| - | - Discussed plans and schedule for next week.
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| - | ===5/20/13===
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| - | LP, XL
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| - | - We performed T7 Mutagen Concentration Test
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| - | : [[5.20 Mutagen Concentration Experiment]]
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| - | - We performed T7 Minor Capsid Protein PCR
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| - | : [[5.20 T7 Minor Capsid Protein PCR]]
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| - | ===5/21/13===
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| - | XL
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| - | - started two 5mL E coli BL21 overnight at around 7:00pm
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| - | ===5/22/13===
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| - | - Continued with [[5.20 Mutagen Concentration Experiment|T7 Mutagen Concentration Test]]
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| - | - Continued with [[5.20 T7 Minor Capsid Protein PCR|T7 Minor Capsid Protein PCR ]] by running an agarose gel to confirm that we got the desired PCR product.
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| | ==June== | | ==June== |
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