Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/PR

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Revision as of 21:56, 28 May 2013

Small Phage Spring Notebook: May 13 - May 26 Progress Report

Overview

Winter

Spring

Summer

Fall

1. Goals for the week

2.Experiments and results

5.9 T7+ Liquid Culture Phage Concentration Test #2

We infected E. coli BL21 with different concentrations (1ul, 10ul, and 100ul) of phage to see which would yield the highest titer. Plaques formed up to -8 on all the spot tests, showing that the concentration difference had no effect on the titer. However, the plates were badly contaminated.

5.13 Determining E. coli Concentration With Spectrophotometer

To check if we could determine E. coli concentration with a spectrophotometer, we created a 7/5 dilution series of E. coli. We then measured the absorption at 600nm of each E. coli dilution. When plotted, these measurements showed a linear relationship between E. coli concentration and the absorption reading.

3. Next Steps

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 <font face="Calibri" size="3">
-'''Add text'''
+'''1. Goals for the week '''
+'''2.Experiments and results '''
+.9 T7+ Liquid Culture Phage Concentration Test #2
+: We infected E. coli BL21 with different concentrations (1ul, 10ul, and 100ul) of phage to see which would yield the highest titer.  Plaques formed up to -8 on all the spot tests, showing that the concentration difference had no effect on the titer.  However, the plates were badly contaminated.
+.13 Determining E. coli Concentration With Spectrophotometer
+: To check if we could determine E. coli concentration with a spectrophotometer, we created a 7/5 dilution series of E. coli.  We then measured the absorption at 600nm of each E. coli dilution.  When plotted, these measurements showed a linear relationship between E. coli concentration and the absorption reading.
+[[File:Spec.png]]
+'''3. Next Steps'''
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