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From 2013.igem.org

Revision as of 11:42, 14 August 2013 (view source) AliceHaworth (Talk | contribs) (Created page with "<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;"> {| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border=...") ← Older edit		Latest revision as of 14:16, 22 August 2013 (view source) AliceHaworth (Talk | contribs) (→Single digest)
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-	<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;">	+	==Single digest==
-	{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"	+	*Digesting RFP plasmid again using a single restriction enzyme- XbaI
-	!align="center"|[[Team:Leicester|Home]]	+	*Digesting DNA samples 1-6
-	!align="center"|[[Team:Leicester/Team|Team]]	+	*200ng of DNA is run
-	!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]	+	*Volumes for digest:
-	!align="center"|[[Team:Leicester/Project|Project]]	+	{| border=1
-	!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]	+	|Sample nr||1||2||3||4||5||6
-	!align="center"|[[Team:Leicester/Modeling|Modeling]]	+	|-
-	!align="center"|[[Team:Leicester/Notebook|Notebook]]	+	|DNA||0.7||0.7||0.8||1.3||1.2||2.6
-	!align="center"|[[Team:Leicester/Safety|Safety]]	+	|-
-	!align="center"|[[Team:Leicester/Attributions|Attributions]]	+	|Buffer||2||2||2||2||2||2
		+	|-
		+	|Water||16.3||16.3||16.2||15.7||15.8||14.4
		+	|-
		+	|XbaI||1||1||1||1||1||1
	|}		|}
-	</div>	+	*Buffer used was cutsmart
		+	*Incubate samples at 37C for 1h
		+	*2ul of orange G dye was added to each samples
		+	*Samples were then frozen overnight to run on gel on Monday

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Latest revision as of 14:16, 22 August 2013

Single digest

• Digesting RFP plasmid again using a single restriction enzyme- XbaI
• Digesting DNA samples 1-6
• 200ng of DNA is run
• Volumes for digest:

Sample nr	1	2	3	4	5	6
DNA	0.7	0.7	0.8	1.3	1.2	2.6
Buffer	2	2	2	2	2	2
Water	16.3	16.3	16.2	15.7	15.8	14.4
XbaI	1	1	1	1	1	1

• Buffer used was cutsmart
• Incubate samples at 37C for 1h
• 2ul of orange G dye was added to each samples
• Samples were then frozen overnight to run on gel on Monday