"
Team:DTU-Denmark/Notebook/29 June 2013
From 2013.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
Made PCRs on pZA21, GFP SF TAT, RFP all samples where made in duplicates. | Made PCRs on pZA21, GFP SF TAT, RFP all samples where made in duplicates. | ||
| - | In tube 1+2, 5+6 where pZA21. | + | *In tube 1+2, 5+6 where pZA21. |
| - | In 9+10 GFP SF TAT | + | *In 9+10 GFP SF TAT |
| - | In 3+4, 7+8 RFP | + | *In 3+4, 7+8 RFP |
First program was 55°C annealing and 2:00 extension time with tube: 1+2, 3+4. | First program was 55°C annealing and 2:00 extension time with tube: 1+2, 3+4. | ||
Revision as of 09:14, 23 August 2013
29 June 2013
Navigate to the Previous or the Next Entry
Contents |
208 lab
Main purposes today
New PCR with x7-polymerase.
who were in the lab
Kristian
Procedure
Made PCRs on pZA21, GFP SF TAT, RFP all samples where made in duplicates.
- In tube 1+2, 5+6 where pZA21.
- In 9+10 GFP SF TAT
- In 3+4, 7+8 RFP
First program was 55°C annealing and 2:00 extension time with tube: 1+2, 3+4. Second program was 64°C annealing and 2:00 extension time with tube: 5+6, 7+8. Last program was 69°C annealing and 1:00 extension time with tube: 9+10.
Results
None of the PCRs worked.
Conclusion from today
...
Navigate to the Previous or the Next Entry
