Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period4/Dailylog

Small Phage

March-April

May-June

July-August

September-October

• Started characterizing post-CsCl phage in 8.14 Mutagen Concentration Test - Seventh Protocol.

• Started approximately 10mL of E coli B liquid culture overnight.

• Streaked out E coli B.

8/18/13

• Continued characterization of post-CsCl phage in 8.14 Mutagen Concentration Test - Seventh Protocol.

• Started approximately 20mL of E coli B liquid culture overnight.

8/19/13

• Plaque test of post-CsCl phage in 8.14 Mutagen Concentration Test - Seventh Protocol.

• Discussed modeling result of 8.16 Modeling Phage Plaque Sizes - Experiment One with Dr. Grose -> need to repeat for T1 and T2 starting from dilution series and spot test.

• Started approximately 30 mL of E coli B liquid culture overnight.

8/20/13

• Selected for 12 small plaques (S1-S12, relatively large phage) and 12 big plaques (B1-B12, relatively small phage) at respective end of spectrum for 8.14 Mutagen Concentration Test - Seventh Protocol. Spectrum ends were chose because the likelihood of contamination between different layers of CsCl during the extraction process.

• Performed dilution series and spot test for T1, T2 (from stock), and T7.

• Started approximately 30 mL of E coli B liquid culture overnight.

8/21/13

• Spot test for T7 only showed plaques up to -5. Might not be high enough for mutagenesis. Need to propagate.

• Plated the S1-S12 and B1-B12 samples.

• Started approximately 6 mL of E coli B liquid culture overnight

8/22/13

• Nothing showed on the S1-S12 and B1-B12 plates. Wildtype had too many plaques (T7 at -4).

• T7 propagation.

• Started approximately 20 mL of E coli liquid culture overnight.

8/7/13

- Performed spot test to estimate phage titerfor 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

- Phage Purification Group started the purification process with CsCl gradient.

8/8/13

- Started approximately 15mL of E coli B liquid culture overnight.

8/9/13

- Performed Preliminary Titer for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

8/11/13

- Started approximately 20mL of E coli B liquid culture overnight.

8/12/13

- Performed titer - repeat for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments based on the results from 8.10 preliminary titer test.

- Started T1 propagation.

- Made accurate x2 top agar as preparation for 8.16 Modeling Phage Plaque Sizes - Experiment One.

8/13/13

- Started approximately 20mL of E coli B liquid culture overnight.

8/14/13

- Performed mutagenesis and spot test for 8.14 Mutagen Concentration Test - Seventh Protocol.

- Performed spot test for T1 propagation. Spotted 5uL of -2 through -7 dilutions.

8/15/13

- T1 propagation revealed plaques on each of the tested dilutions, suggesting that this propagated T1 phage stock has a titer of at least 10⁹ pfu/mL -> more accurate spot test / titer needed to determine the exact phage concentration.

- Started approximately 30mL of E coli B liquid culture overnight.

8/16/13

- Started 8.16 Modeling Phage Plaque Sizes - Experiment One.

- Phage Purification team performed the CsCl gradient for 8.14 Mutagen Concentration Test - Seventh Protocol.