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Team:Groningen/Labwork/27 August 2013
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<h2>Inne</h2> | <h2>Inne</h2> | ||
| + | Did a mini prep of the colonies with biobrick BBa_k1085006, samples s7,s8,s11,s12,s13,s14. 30 uL elution buffer was used instead of 50 uL. Determined the concentration of the samples via nanodrop, also nanodropped the samples Sander miniprepped. | ||
<table border="1"> | <table border="1"> | ||
<tr> | <tr> | ||
| Line 29: | Line 30: | ||
<tr> | <tr> | ||
<td>s3</td> | <td>s3</td> | ||
| - | <td>75.6</td> | + | <td>75.6 ng/L</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>s4</td> | <td>s4</td> | ||
| - | <td>93.3</td> | + | <td>93.3 ng/L</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>s9</td> | <td>s9</td> | ||
| - | <td>87.9</td> | + | <td>87.9 ng/L</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>s10</td> | <td>s10</td> | ||
| - | <td>79.4</td> | + | <td>79.4 ng/L</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>s7</td> | <td>s7</td> | ||
| - | <td> | + | <td>91.6 ng/L</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>s8</td> | <td>s8</td> | ||
| - | <td> | + | <td>82.8 ng/L</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>s11</td> | <td>s11</td> | ||
| - | <td> | + | <td>94.8 ng/L</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>s12</td> | <td>s12</td> | ||
| - | <td> | + | <td>86.6 ng/L</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>s13</td> | <td>s13</td> | ||
| - | <td> | + | <td>105.6 ng/L</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>s14</td> | <td>s14</td> | ||
| - | <td> | + | <td>104.5 ng/L</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| + | <br> Inoculated the motility assay plate with wt, delta cheY and delta cheY/des. All strains will be grown in duplo overnight at 37C and 30C, time of inoculation: 18:20. | ||
| + | |||
| + | <h2>Sander</h2> | ||
| + | Did a mini prep of the colonies of samples s3,s4,s9,s10. | ||
| + | Did a restriction digest of the samples and ran them over a 0,8% agarose gel. the samples were not present in the plasmids. | ||
| + | |||
| + | <h2>Sebas</h2> | ||
| + | Designed and ordered primers to turn around the hy_spank promoter to end up with our final (correct) backbone: | ||
| + | <img src="https://static.igem.org/mediawiki/2013/5/5f/BBa_K1085000.jpg" width="500" height="500"></img> | ||
| + | <br>Found another backbone (pX), not biobrick compatible but with sites SpeI*SdaI (same overhang as XbaI*PstI). | ||
</div> | </div> | ||
Latest revision as of 08:19, 30 August 2013
Inne
Did a mini prep of the colonies with biobrick BBa_k1085006, samples s7,s8,s11,s12,s13,s14. 30 uL elution buffer was used instead of 50 uL. Determined the concentration of the samples via nanodrop, also nanodropped the samples Sander miniprepped.| Sample | Concentration |
| s3 | 75.6 ng/L |
| s4 | 93.3 ng/L |
| s9 | 87.9 ng/L |
| s10 | 79.4 ng/L |
| s7 | 91.6 ng/L |
| s8 | 82.8 ng/L |
| s11 | 94.8 ng/L |
| s12 | 86.6 ng/L |
| s13 | 105.6 ng/L |
| s14 | 104.5 ng/L |
Inoculated the motility assay plate with wt, delta cheY and delta cheY/des. All strains will be grown in duplo overnight at 37C and 30C, time of inoculation: 18:20.
Sander
Did a mini prep of the colonies of samples s3,s4,s9,s10. Did a restriction digest of the samples and ran them over a 0,8% agarose gel. the samples were not present in the plasmids.Sebas
Designed and ordered primers to turn around the hy_spank promoter to end up with our final (correct) backbone:
Found another backbone (pX), not biobrick compatible but with sites SpeI*SdaI (same overhang as XbaI*PstI).
iGEM 2013 Groningen
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