29/08/13

From 2013.igem.org

(Difference between revisions)
(→Run Tod operon ligation genes on the gel)
Line 5: Line 5:
==Isolate the DNA from gel==
==Isolate the DNA from gel==
*DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol
*DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol
 +
 +
==Measuring concentration of DNA using nanodrop==
 +
{|border=1
 +
|Sample||Volume ul||Concentration ng/ul||260/280||260/230
 +
|-
 +
|A1||30||14.7||1.81||1.41
 +
|-
 +
|A2||31||13||1.71||1.43
 +
|-
 +
|B1||30||23.6||1.93||1.02
 +
|-
 +
|B2||29||21.9||1.88||1.33
 +
|-
 +
|C1||29||14.7||1.83||0.63
 +
|-
 +
|C2||30||15.5||1.86||0.64
 +
|}

Revision as of 10:19, 30 August 2013

Contents

Make 2% agarose gel

Run Tod operon ligation genes on the gel

  • 1.5h at 70V

Isolate the DNA from gel

  • DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol

Measuring concentration of DNA using nanodrop

SampleVolume ulConcentration ng/ul260/280260/230
A13014.71.811.41
A231131.711.43
B13023.61.931.02
B22921.91.881.33
C12914.71.830.63
C23015.51.860.64
Retrieved from "http://2013.igem.org/29/08/13"