Team:BYU Provo/Notebook/Phage Purification/Springexp

From 2013.igem.org

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{| width="100%"
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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage March - April Notebook'''</font>
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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook'''</font>
<br>
<br>
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<font color="#333399" size="3" font face="Calibri">
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: [[Team:BYU_Provo/Phage_Purification|Overview]]
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: [[Team:BYU_Provo/Small_Phage|Overview]]
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: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
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: [[Team:BYU Provo/Notebook/Phage_Purification/Springexp|May-June]]
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: [[Team:BYU Provo/Notebook/SmallPhage/Springexp|May-June]]
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: [[Team:BYU Provo/Notebook/Phage_Purification/Summerexp|July-August]]
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: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|July-August]]
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: [[Team:BYU Provo/Notebook/Phage_Purification/Fallexp|September-October]]
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: [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|September-October]]
</font>
</font>
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| style="width: 50%; background-color: transparent;"|
| style="width: 50%; background-color: transparent;"|
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<font size="5" font face="Calibri"> '''March 15 - March 31''' </font>
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<font size="5" font face="Calibri"> '''May 1 - May 12''' </font>
<br>  
<br>  
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<font size="3" font face="Calibri">  
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<font size="3" font face="Calibri"> This is the start of Spring Term at BYU! The weather is finally getting better: no more snowing. And we are ready to do some serious lab work! While we are waiting for our mutgen, 5-bromodeoxyuridine, to arrive, we decided to start this term with establishing the basic procedures for our direct evolution method. Specifically, this involves making sure that we can (1) grow and amplify T7 phage in liquid culture, (2) select for smaller phage using concentrated top agar, and (3) amplify phage from a single plaque. During these two weeks, we also designed primers for cloning and sequencing T7 capsid proteins as well as mapped out detailed procedures for both cloning T7 capsid proteins and applying mutagen. </font>
-
This marks the start of our small phage group! Although we are still on the background-research phase and not doing any wet-lab experiments, we are sure our work will be worthwhile. As Dr. Grose, our wonderful instructor, says "a month in the lab will say you a day in the library." After two weeks of hardcore literature search, we now have a clear idea of where our project is headed. And we are super excited to get started!
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</font>
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<font color="#333399" size="4" font face="Calibri">
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/PR| Progress Report]] </font>
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<br>
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| style="width: 20%; background-color: transparent;"| [[File:Spring1.JPG|250px|center]]
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|-
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| style="width: 18%; background-color: transparent;"|
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| <font size="5" font face="Calibri"> '''May 13 - May 26''' </font>
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<br>
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<font size="3" font face="Calibri"> In these two weeks, we performed our first round of mutagenesis and first PCR. Although we are still testing the results to optimize our protocol, we are optimistic about the outcome!</font>
<br>
<br>
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<font color="#333399" size="4" font face="Calibri">
<font color="#333399" size="4" font face="Calibri">
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period5/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period2/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period5/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period2/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period5/PR|Presentation]] </font>
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period2/PR| Progress Report]] </font>
<br>
<br>
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| style="width: 20%; background-color: transparent;"| [[File:Winter1.JPG|250px|center]]
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| style="width: 20%; background-color: transparent;"| [[File:Spring2.JPG|250px|center]]
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|-
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| style="width: 18%; background-color: transparent;"|
| style="width: 18%; background-color: transparent;"|
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| <font size="5" font face="Calibri"> '''April 1 - April 14''' </font>
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| <font size="5" font face="Calibri"> '''May 27 - June 9''' </font>
<br>  
<br>  
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<font size="3" font face="Calibri"> Add description of the two weeks </font>
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<font size="3" font face="Calibri"> Add description! </font>
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<font color="#333399" size="4" font face="Calibri">
<font color="#333399" size="4" font face="Calibri">
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period6/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period6/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period6/PR| Progress Report]] </font>
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/PR| Progress Report]] </font>
<br>
<br>
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| style="width: 20%; background-color: transparent;"| [[File:Winter2.JPG|250px|center]]
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| style="width: 20%; background-color: transparent;"| [[File:Spring3.JPG|250px|center]]
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| style="width: 18%; background-color: transparent;"|
| style="width: 18%; background-color: transparent;"|
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| <font size="5" font face="Calibri"> '''May 27 - June 9''' </font>
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| <font size="5" font face="Calibri"> '''June 10 - June 23''' </font>
<br>  
<br>  
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<font size="3" font face="Calibri"> Add description of the two weeks! </font>
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<font size="3" font face="Calibri"> Add description! </font>
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<br>
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<font color="#333399" size="4" font face="Calibri">
<font color="#333399" size="4" font face="Calibri">
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period7/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period4/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period7/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period4/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period7/PR| Progress Report]] </font>
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period4/PR| Progress Report]] </font>
<br>
<br>
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| style="width: 20%; background-color: transparent;"| [[File:Winter3.JPG|250px|center]]
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| style="width: 20%; background-color: transparent;"| [[File:Spring4.JPG|250px|center]]
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|-
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| style="width: 18%; background-color: transparent;"|
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| <font size="5" font face="Calibri"> '''June 24 - June 30''' </font>
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<br>
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 +
<font size="3" font face="Calibri"> Add description! </font>
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<br>
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<font color="#333399" size="4" font face="Calibri">
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[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period5/Dailylog|Daily log]]
 +
 
 +
[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period5/Explist|Experiment Listing]]
 +
 
 +
[[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period5/PR| Progress Report]] </font>
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<br>
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| style="width: 20%; background-color: transparent;"| [[File:Winter2.JPG|250px|center]]

Revision as of 20:00, 3 June 2013


Small Phage May - June Notebook



Overview
March-April
May-June
July-August
September-October

May 1 - May 12


This is the start of Spring Term at BYU! The weather is finally getting better: no more snowing. And we are ready to do some serious lab work! While we are waiting for our mutgen, 5-bromodeoxyuridine, to arrive, we decided to start this term with establishing the basic procedures for our direct evolution method. Specifically, this involves making sure that we can (1) grow and amplify T7 phage in liquid culture, (2) select for smaller phage using concentrated top agar, and (3) amplify phage from a single plaque. During these two weeks, we also designed primers for cloning and sequencing T7 capsid proteins as well as mapped out detailed procedures for both cloning T7 capsid proteins and applying mutagen.


Daily log

Experiment Listing

Progress Report


Spring1.JPG


May 13 - May 26


In these two weeks, we performed our first round of mutagenesis and first PCR. Although we are still testing the results to optimize our protocol, we are optimistic about the outcome!


Daily log

Experiment Listing

Progress Report



Spring2.JPG


May 27 - June 9


Add description!


Daily log

Experiment Listing

Progress Report



Spring3.JPG


June 10 - June 23


Add description!


Daily log

Experiment Listing

Progress Report



Spring4.JPG
June 24 - June 30


Add description!


Daily log

Experiment Listing

Progress Report



Winter2.JPG

Retrieved from "http://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp"