Exeter/29 August 2013

From 2013.igem.org

(Difference between revisions)
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==Digestion==
==Digestion==
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{| class="wikitable"
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|-
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! Part !! K592011 (Part A) !! B0015 (Part B) !! AMP Plasmid !! RFP (Control)
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|-
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| DNA || 1.52 ul || 6.63 ul || 10.0 ul || 4.05 ul
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|-
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| Non-nuclease Water || 14.48 ul || 9.37 ul || 5.5 ul || 11.95 ul
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|-
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| NEB Buffer || 2.5 ul || 2.5 ul || 2.5 ul || 2.5 ul
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|-
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| BSA || 0.5 ul || 0.5 ul || 0.5 ul || 0.5 ul
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|-
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| EcoR1 || 0.5 ul || - || 0.5 ul || 0.5 ul
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|-
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| Spe1 || 0.5 || - || - || -
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|-
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| Xbal1 || - || 0.5 ul || - || -
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|-
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| Pst1 || - || 0.5 ul || 0.5 ul || 0.5 ul
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|-
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| Dpn1 || - || - || 0.5 ul || -
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|}

Revision as of 11:18, 2 September 2013

Minipreps

We miniprepped:

  • 1 + 4
  • K592011 B
  • K864404
  • Cph8 #3
  • 2 + 4
  • K592022 B
  • ENvZ no antibiotic
  • ENvZ
  • Cph8 #5


The liquid cultures of K592011 A and K592022 A did not grow.


NanoDrop

The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.

  • K592011 B - 164.2 ng/ul
  • B0015 - 37.7 ng/ul
  • OmpR prom - 50.7 ng/ul
  • RBS - 49.1 ng/ul


Digestion

Part K592011 (Part A) B0015 (Part B) AMP Plasmid RFP (Control)
DNA 1.52 ul 6.63 ul 10.0 ul 4.05 ul
Non-nuclease Water 14.48 ul 9.37 ul 5.5 ul 11.95 ul
NEB Buffer 2.5 ul 2.5 ul 2.5 ul 2.5 ul
BSA 0.5 ul 0.5 ul 0.5 ul 0.5 ul
EcoR1 0.5 ul - 0.5 ul 0.5 ul
Spe1 0.5 - - -
Xbal1 - 0.5 ul - -
Pst1 - 0.5 ul 0.5 ul 0.5 ul
Dpn1 - - 0.5 ul -


Part K592011 (Part A) B0015 (Part B) AMP Plasmid RFP (Control)
DNA 1.52 ul 6.63 ul 10.0 ul 4.05 ul
Non-nuclease Water 14.48 ul 9.37 ul 5.5 ul 11.95 ul
NEB Buffer 2.5 ul 2.5 ul 2.5 ul 2.5 ul
BSA 0.5 ul 0.5 ul 0.5 ul 0.5 ul
EcoR1 0.5 ul - 0.5 ul 0.5 ul
Spe1 0.5 - - -
Xbal1 - 0.5 ul - -
Pst1 - 0.5 ul 0.5 ul 0.5 ul
Dpn1 - - 0.5 ul -
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