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Exeter/29 August 2013
From 2013.igem.org
(Difference between revisions)
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{| class="wikitable" | {| class="wikitable" | ||
|- | |- | ||
| - | ! Part !! | + | ! Part !! OmpR (Part A) !! RBS (Part B) !! AMP Plasmid !! RFP (Control) |
|- | |- | ||
| - | | DNA || | + | | DNA || 4.93 ul || 5.09 ul || 10.0 ul || 3.30 ul |
|- | |- | ||
| - | | Non-nuclease Water || | + | | Non-nuclease Water || 11.07 ul || 10.91 ul || 5.5 ul || 12.70 ul |
|- | |- | ||
| NEB Buffer || 2.5 ul || 2.5 ul || 2.5 ul || 2.5 ul | | NEB Buffer || 2.5 ul || 2.5 ul || 2.5 ul || 2.5 ul | ||
Revision as of 11:20, 2 September 2013
Minipreps
We miniprepped:
- 1 + 4
- K592011 B
- K864404
- Cph8 #3
- 2 + 4
- K592022 B
- ENvZ no antibiotic
- ENvZ
- Cph8 #5
The liquid cultures of K592011 A and K592022 A did not grow.
NanoDrop
The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.
- K592011 B - 164.2 ng/ul
- B0015 - 37.7 ng/ul
- OmpR prom - 50.7 ng/ul
- RBS - 49.1 ng/ul
Digestion
| Part | K592011 (Part A) | B0015 (Part B) | AMP Plasmid | RFP (Control) |
|---|---|---|---|---|
| DNA | 1.52 ul | 6.63 ul | 10.0 ul | 4.05 ul |
| Non-nuclease Water | 14.48 ul | 9.37 ul | 5.5 ul | 11.95 ul |
| NEB Buffer | 2.5 ul | 2.5 ul | 2.5 ul | 2.5 ul |
| BSA | 0.5 ul | 0.5 ul | 0.5 ul | 0.5 ul |
| EcoR1 | 0.5 ul | - | 0.5 ul | 0.5 ul |
| Spe1 | 0.5 | - | - | - |
| Xbal1 | - | 0.5 ul | - | - |
| Pst1 | - | 0.5 ul | 0.5 ul | 0.5 ul |
| Dpn1 | - | - | 0.5 ul | - |
| Part | OmpR (Part A) | RBS (Part B) | AMP Plasmid | RFP (Control) |
|---|---|---|---|---|
| DNA | 4.93 ul | 5.09 ul | 10.0 ul | 3.30 ul |
| Non-nuclease Water | 11.07 ul | 10.91 ul | 5.5 ul | 12.70 ul |
| NEB Buffer | 2.5 ul | 2.5 ul | 2.5 ul | 2.5 ul |
| BSA | 0.5 ul | 0.5 ul | 0.5 ul | 0.5 ul |
| EcoR1 | 0.5 ul | - | 0.5 ul | 0.5 ul |
| Spe1 | 0.5 | - | - | - |
| Xbal1 | - | 0.5 ul | - | - |
| Pst1 | - | 0.5 ul | 0.5 ul | 0.5 ul |
| Dpn1 | - | - | 0.5 ul | - |
