02/09/13

From 2013.igem.org

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==Preparation of IPTG and Chloramphenicol plates==
 +
*The IPTG/Chloramphenicol plates that were made earlier on for the volatile organic compound (VOC) test were found to be contaminated.
 +
* Hence new plates had to be made using the below protocol;
 +
 +
PROTOCOL:
 +
* x2 400ml of already prepared and sterilized agar was obtained from the media kitchen.
 +
* The agar was microwaved using the lowest power level at 2-3 mins intervals until completely melted.
 +
* The x2 400ml agar were then placed in the 37 degrees room to cool.
 +
* After cooling, 800ul of cholramphenicol and 400ul of IPTG were added to one bottle whilst only 800ul of chloramphenicol was added to the second bottle.
 +
* The contents of both agar bottles was then poured into ...

Revision as of 11:58, 2 September 2013

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Preparation of IPTG and Chloramphenicol plates

  • The IPTG/Chloramphenicol plates that were made earlier on for the volatile organic compound (VOC) test were found to be contaminated.
  • Hence new plates had to be made using the below protocol;

PROTOCOL:

  • x2 400ml of already prepared and sterilized agar was obtained from the media kitchen.
  • The agar was microwaved using the lowest power level at 2-3 mins intervals until completely melted.
  • The x2 400ml agar were then placed in the 37 degrees room to cool.
  • After cooling, 800ul of cholramphenicol and 400ul of IPTG were added to one bottle whilst only 800ul of chloramphenicol was added to the second bottle.
  • The contents of both agar bottles was then poured into ...
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